Total number of subtomograms

[Reza Khayat]

Hi,

What is the minimum number of total subtomograms that should be used during the training? Is this independent/dependent of the total number of tomograms? 

Instructions are:  "Usually total 300 subtomograms are sufficient for IsoNet refine." Is this total of all tomograms? Thanks.

Best wishes,

Reza

[YUNTAO LIU]

Hi Reza,

This is the total of all tomograms. I guess more than 100 subtomos in total might be the minimum for training. For simulated data, I tried 20 apoferritin simulated subtomograms and the results are already good enough.  

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Best Regards,

Yuntao Liu,  Postdoc.

California NanoSystem Institute

University of California Los Angeles