Hey Kristoffer,
I imported my Data from Salmon via Tximeta, then used isoformSwitchAnalysisPart1, but got an error. Here is my script:
salmonDf <- prepareSalmonFileDataFrame("salmonoutput")
salmonDf$condition <- c('FXS','FXS','Control','Control','FXS','FXS','FXS','FXS','Control','Control','Control','Control','Control')
aSwitchList <- importSalmonData(
salmonFileDataFrame = salmonDf,
showProgress=FALSE)
aslist <- isoformSwitchAnalysisPart1(switchAnalyzeRlist=aSwitchList,
pathToOutput = '/isoformsalmontximeta',
outputSequences = TRUE,
prepareForWebServers = TRUE)
The error: Error in isoformSwitchAnalysisPart1(switchAnalyzeRlist = aSwitchList, :
When ORFs are not annotated you must supply the GTF with known annotation to the 'pathToGTF' argument.
Could you please help me?
Best,
Lu