Error "no repilcate to estimate"

[pshtiwan babane]

Hello Everyone

after updating isoformswitchAnalzerR Package, i got this error and my design includes 6 condition but aSwitchList function only do for condition 1 and condition 3. again this happen after updating the packages.

aSwitchListAnalyzed <- isoformSwitchTestDEXSeq( + switchAnalyzeRlist = aSwitchListFiltered, + reduceToSwitchingGenes=TRUE + ) Step 1 of 2: Testing each pairwise comparisons with DEXSeq (this might be a bit slow)... Estimated run time is: 182.8 min | | 0%Error: BiocParallel errors 1 remote errors, element index: 1 0 unevaluated and other errors first remote error: Error in estimateDispersionsGeneEst(x, maxit = maxit, quiet = quiet, modelMatrix = modelMatrix, : the number of samples and the number of model coefficients are equal, i.e., there are no replicates to estimate the dispersion. use an alternate design formula In addition: Warning message: In isoformSwitchTestDEXSeq(switchAnalyzeRlist = exampleSwitchListFiltered, : You seem to have many replicates. We therfore recomend you use isoformSwitchTestSatuRn() instead. It is just as accurate and much faster - especially for larger datasets.
Best regards