IGB depth graph for customer genomes

[Xiao Lei]

Dear IGB community,

Is there a way to show depth graph for customer genomes? I can load the genome and sorted bam file into IGB without problem, but all the options under "Graph" tab are greyed out (attached image), I would like to see depth graph of the genome, is there a way I can do? 

Thanks ahead,

Xiao

[Nowlan Freese]

Hi Xiao,

You can create a depth graph from a bam file using IGB Track Operations. 

To create a depth graph:

Right-click on the track's label on the left side of IGB (CLIP33BC1_M1T.sorted.bam in your example).

Highlight Track Operations in the right-click menu and select Depth Graph (All).

This will create a new track with the depth graph information. 

Additional information can be found in the IGB User's Guide here: https://wiki.bioviz.org/confluence/display/igbman/Creating+graph+tracks+from+annotations+tracks

Let me know if this helps or if you have any other questions!

Best,

Nowlan