Nature Methods Contents: March 2007 Volume 4 Number 3, pp 189 - 293

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March 2007, Volume 4 No 3 In This Issue Editorial Correspondence Research Highlights News and Views Perspective Brief Communications Articles Protocols Technology Feature Application Note ADVERTISEMENT This alert is supported by Abcam Don't be a cowboy, use a loading control! Loading controls are essential for proper interpretation of western blots. Abcam’s range includes beta Actin, GAPDH and Tubulin antibodies as well as Mitochondrial loading controls, all selected for consistently high quality results in western blot. Check out the loading controls Abwire today methagora - the commenting forum from Nature Methods is now live. Read and participate in community discussions on important and controversial methodological topics from imaging to RNAi, highlighted by the editors of Nature Methods. Nature Publishing Group's new site for authors and peer-reviewers is at www.nature.com/authors. Authors & referees @ npg is a central resource for information about Nature Publishing Group's publication policies, author/referee services and benefits, as well as publishing news and feedback for and from our community of authors, peer-reviewers, readers and other visitors. www.nature.com/authors In This Issue Top PDF Editorial Top Social software p189 doi:10.1038/nmeth0307-189 Abstract | Full Text | PDF Correspondence Top Same computational analysis, different miRNA target predictions p191 Xiequn Xu doi:10.1038/nmeth0307-191a Full Text | PDF Same computational analysis, different miRNA target predictions p191 Artemis G Hatzigeorgiou doi:10.1038/nmeth0307-191b Full Text | PDF Primer-site SNPs mask mutations p192 Aaron R Quinlan & Gabor T Marth doi:10.1038/nmeth0307-192 Full Text | PDF Research Highlights Top Absolute proteomics p195 Allison Doerr doi:10.1038/nmeth0307-195 Abstract | Full Text | PDF Making mice at high speed pp196 - 197 Nicole Rusk doi:10.1038/nmeth0307-196a Abstract | Full Text | PDF Human mice pp196 - 197 Nicole Rusk doi:10.1038/nmeth0307-196b Abstract | Full Text | PDF News in brief p197 doi:10.1038/nmeth0307-197 Full Text | PDF Unraveling transcriptional networks p198 Jesse Potash doi:10.1038/nmeth0307-198 Abstract | Full Text | PDF Human chromatin at the FAIRE p200 Natalie de Souza doi:10.1038/nmeth0307-200 Abstract | Full Text | PDF The dynamic proteasome pp202 - 203 Irene Kaganman doi:10.1038/nmeth0307-202a Abstract | Full Text | PDF Trapped in interaction pp202 - 203 Natalie de Souza doi:10.1038/nmeth0307-202b Abstract | Full Text | PDF Automatically screen all of your CHO-S, NSO, HEK 293 cells or hybridomas - ClonePix FL lets you identify and isolate your most valuable cell lines, singling out the highest producers and the most stable cell lines for dependable productivity in a fraction of the time. Discover your star cell line at light speed. Make contact at www.genetix.com/clonepix News and Views Top The unnatural culture of amino acids pp205 - 206 Corinne D Hausmann, Jiqiang Ling & Michael Ibba doi:10.1038/nmeth0307-205 Abstract | Full Text | PDF Perspective Top Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry pp207 - 214 Joshua E Elias & Steven P Gygi doi:10.1038/nmeth1019 Abstract | Full Text | PDF Brief Communications Top Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy pp215 - 217 Michael Marko, Chyongere Hsieh, Richard Schalek, Joachim Frank & Carmen Mannella doi:10.1038/nmeth1014 Abstract | Full Text | PDF Imaging receptor microdomains on leukocyte subsets in live mice pp219 - 222 Elaine Y Chiang, Andres Hidalgo, Jungshan Chang & Paul S Frenette doi:10.1038/nmeth1018 Abstract | Full Text | PDF Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection pp223 - 225 Christopher Deufel, Scott Forth, Chad R Simmons, Siavash Dejgosha & Michelle D Wang doi:10.1038/nmeth1013 Abstract | Full Text | PDF The development of a bioengineered organ germ method pp227 - 230 Kazuhisa Nakao, Ritsuko Morita, Yasumitsu Saji, Kentaro Ishida, Yusuke Tomita, Miho Ogawa, Masahiro Saitoh, Yasuhiro Tomooka & Takashi Tsuji doi:10.1038/nmeth1012 Abstract | Full Text | PDF Articles Top Reproducible isolation of distinct, overlapping segments of the phosphoproteome pp231 - 237 Bernd Bodenmiller, Lukas N Mueller, Markus Mueller, Bruno Domon & Ruedi Aebersold doi:10.1038/nmeth1005 Abstract | Full Text | PDF Genetic incorporation of unnatural amino acids into proteins in mammalian cells pp239 - 244 Wenshe Liu, Ansgar Brock, Shuo Chen, Shuibing Chen & Peter G Schultz doi:10.1038/nmeth1016 Abstract | Full Text | PDF Ubc9 fusion-directed SUMOylation (UFDS): a method to analyze function of protein SUMOylation pp245 - 250 Astrid Jakobs, Jesko Koehnke, Fabian Himstedt, Martin Funk, Bernhard Korn, Matthias Gaestel & Rainer Niedenthal doi:10.1038/nmeth1006 Abstract | Full Text | PDF Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC pp251 - 256 Mamie Z Li & Stephen J Elledge doi:10.1038/nmeth1010 Abstract | Full Text | PDF Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology pp257 - 262 Yasumasa Mitani, Alexander Lezhava, Yuki Kawai, Takeshi Kikuchi, Atsuko Oguchi-Katayama, Yasushi Kogo, Masayoshi Itoh, Toru Miyagi, Hideki Takakura, Kanako Hoshi, Chiaki Kato, Takahiro Arakawa, Kazuhiro Shibata, Kenji Fukui, Ryoji Masui, Seiki Kuramitsu, Kazuma Kiyotani, Alistair Chalk, Katsuhiko Tsunekawa, Masami Murakami, Tetsuya Kamataki, Takanori Oka, Hiroshi Shimada, Paul E Cizdziel & Yoshihide Hayashizaki doi:10.1038/nmeth1007 Abstract | Full Text | PDF A retroviral strategy that efficiently creates chromosomal deletions in mammalian cells pp263 - 268 Melanie Bilodeau, Simon Girard, Josee Hebert & Guy Sauvageau doi:10.1038/nmeth1011 Abstract | Full Text | PDF Protocols Top Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target pp269 - 279 Christian Zahnd, Patrick Amstutz & Andreas Pluckthun doi:10.1038/nmeth1003 Abstract | Full Text | PDF Eukaryotic ribosome display with in situ DNA recovery pp281 - 288 Mingyue He & Michael J Taussig doi:10.1038/nmeth1001 Abstract | Full Text | PDF Technology Feature Top Taking on chromatography's hard cases pp289 - 293 Alan Dove doi:10.1038/nmeth0307-289 Abstract | Full Text | PDF Application Note Top Rapid selection of mammalian cells secreting large amounts of therapeutic proteins or peptides  Edmund Newman & Christopher J Mann Abstract | Full Text | PDF Top Nature Protocols is THE online resource for authoritative, peer-reviewed protocols. Ensure you stay up to date with the latest protocols in your discipline by signing up for your FREE e-alerts. ADVERTISEMENT   Director Biotechnology Macquarie University Sydney, Australia Associate Director Biotechnology|Cancer Research / Oncology|Drug Discovery|Molecular Biology Genentech South San Francisco, CA United States Scientist-Immunology Diagnostics Bioinformatics|Biotechnology|Drug Discovery|Immunology Genentech South San Francisco, CA United States Senior Program Specialist / Program Officer Biotechnology|Genetics|Genomics|Molecular Biology|Policy & Social Sciences| International Development Research Centre / Centre de Recherches pour le Developpement International Ottawa, ON Canada Editorial Intern Position Biotechnology|Genetics|Immunology|Molecular Biology|Neuroscience| Nature Publishing Group New York, NY United States Researcher Biomedical Sciences|Biotechnology|Public Health|Medical Sciences|Clinical Medicine| Institut National De La Sant Et De La Recherche Medicale Paris Cedex, France More Naturejobs More Nature Products More Nature Events

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