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An isothermal primer extension method for whole genome amplification of fresh and degraded DNA: applications in comparative genomic hybridization, genotyping and mutation screeningpp2185 - 2194
Cheryl I P Lee et al. Published online: 14 December 2006 | doi:10.1038/nprot.2006.398 Abstract | Full Text | PDF (1,042K)
Xenopus laevis transgenesis by sperm nuclear injectionpp2195 - 2203
Stuart J Smith et al. Published online: 14 December 2006 | doi:10.1038/nprot.2006.325 Abstract | Full Text | PDF (412K)
Genomic systematic evolution of ligands by exponential enrichment (Genomic SELEX) for the identification of protein-binding RNAs independent of their expression levelspp2204 - 2212
Christina Lorenz, Frederike von Pelchrzim and Renee Schroeder Published online: 14 December 2006 | doi:10.1038/nprot.2006.372 Abstract | Full Text | PDF (281K)
Verification of automated peptide identifications from proteomic tandem mass spectrapp2213 - 2222
David L Tabb, David B Friedman and Amy-Joan L Ham Published online: 14 December 2006 | doi:10.1038/nprot.2006.330 Abstract | Full Text | PDF (496K)
Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalenepp2223 - 2226
Andreas K Nussler et al. Published online: 14 December 2006 | doi:10.1038/nprot.2006.341 Abstract | Full Text | PDF (138K)
A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtiipp2227 - 2230
Catherine B Mason, Terry M Bricker and James V Moroney Published online: 14 December 2006 | doi:10.1038/nprot.2006.348 Abstract | Full Text | PDF (273K)
Isolation of Caenorhabditis elegans gene knockouts by PCR screening of chemically mutagenized librariespp2231 - 2240
Giovanni M Lesa Published online: 14 December 2006 | doi:10.1038/nprot.2006.345 Abstract | Full Text | PDF (2,035K)
PEGylation of native disulfide bonds in proteinspp2241 - 2252
Steve Brocchini et al. Published online: 14 December 2006 | doi:10.1038/nprot.2006.346 Abstract | Full Text | PDF (395K)
Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo redpp2253 - 2256
Arthur F J Ram and Frans M Klis Published online: 14 December 2006 | doi:10.1038/nprot.2006.397 Abstract | Full Text | PDF (191K)
Recombinant protein purification by self-cleaving aggregation tagpp2257 - 2262
Wan-Yi Wu et al. Published online: 14 December 2006 | doi:10.1038/nprot.2006.314 Abstract | Full Text | PDF (218K)
Collection of lymph-borne dendritic cells in the ratpp2263 - 2270
Simon WF Milling, Christopher Jenkins and Gordon MacPherson Published online: 21 December 2006 | doi:10.1038/nprot.2006.315 Abstract | Full Text | PDF (512K)
Estimation of baculovirus titer based on viable cell sizepp2271 - 2276
Vasantharajan Janakiraman, William F Forrest and Somasekar Seshagiri Published online: 21 December 2006 | doi:10.1038/nprot.2006.387 Abstract | Full Text | PDF (190K)
Administration and interpretation of the Trail Making Testpp2277 - 2281
Christopher R Bowie and Philip D Harvey Published online: 21 December 2006 | doi:10.1038/nprot.2006.390 Abstract | Full Text | PDF (134K)
Determination of plant resistance to carbamate herbicidal compounds inhibiting cell division and early growth by seed and plantlets bioassayspp2282 - 2287
Yael Weiss et al. Published online: 21 December 2006 | doi:10.1038/nprot.2006.380 Abstract | Full Text | PDF (335K)
Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic-endothelial sinusoid-like structurespp2288 - 2296
Yaakov Nahmias and David J Odde Published online: 21 December 2006 | doi:10.1038/nprot.2006.386 Abstract | Full Text | PDF (371K)
Chemical cleavage of mismatch (CCM) to locate base mismatches in heteroduplex DNApp2297 - 2304
Tania Tabone et al. Published online: 21 December 2006 | doi:10.1038/nprot.2006.352 Abstract | Full Text | PDF (363K)
Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extractspp2305 - 2314
Eva Hannak and Rebecca Heald Published online: 21 December 2006 | doi:10.1038/nprot.2006.396 Abstract | Full Text | PDF (291K)
Clonogenic assay of cells in vitropp2315 - 2319
Nicolaas A P Franken et al. Published online: 21 December 2006 | doi:10.1038/nprot.2006.339 Abstract | Full Text | PDF (195K)
A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromidepp2320 - 2325
G C Allen et al. Published online: 21 December 2006 | doi:10.1038/nprot.2006.384 Abstract | Full Text | PDF (179K)
Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tagspp2326 - 2333
Jose Arnau, Conni Lauritzen and John Pedersen Published online: 21 December 2006 | doi:10.1038/nprot.2006.388 Abstract | Full Text | PDF (224K)
Cell culture and infection system for hepatitis C viruspp2334 - 2339
Takanobu Kato et al. Published online: 21 December 2006 | doi:10.1038/nprot.2006.395 Abstract | Full Text | PDF (202K)
High-throughput screening of activity and enantioselectivity of esterasespp2340 - 2343
Dominique Bottcher and Uwe T Bornscheuer Published online: 21 December 2006 | doi:10.1038/nprot.2006.391 Abstract | Full Text | PDF (204K)
A method for the covalent capture and screening of diverse small molecules in a microarray formatpp2344 - 2352
James E Bradner, Olivia M McPherson and Angela N Koehler Published online: 21 December 2006 | doi:10.1038/nprot.2006.282 Abstract | Full Text | PDF (315K)
DNA methylation: Bisulphite modification and analysispp2353 - 2364
Susan J Clark et al. Published online: 21 December 2006 | doi:10.1038/nprot.2006.324 Abstract | Full Text | PDF (557K)
Flow cytometry and FISH to measure the average length of telomeres (flow FISH)pp2365 - 2376
Gabriela M Baerlocher, Irma Vulto, Gary de Jong and Peter M Lansdorp Published online: 21 December 2006 | doi:10.1038/nprot.2006.263 Abstract | Full Text | PDF (368K)
Synthesis of non-natural ManNAc analogs for the expression of thiols on cell-surface sialic acidspp2377 - 2385
Srinivasa-Gopalan Sampathkumar, Adrienne V Li and Kevin J Yarema Published online: 21 December 2006 | doi:10.1038/nprot.2006.319 Abstract | Full Text | PDF (287K)
Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrayspp2386 - 2398
Souvik Chattopadhaya, Lay Pheng Tan and Shao Q Yao Published online: 21 December 2006 | doi:10.1038/nprot.2006.338 Abstract | Full Text | PDF (481K)
Time-lapse imaging of dendritic spines in vitropp2399 - 2405
J Martin Verkuyl and Andrew Matus Published online: 21 December 2006 | doi:10.1038/nprot.2006.357 Abstract | Full Text | PDF (306K)
Culturing hippocampal neuronspp2406 - 2415
Stefanie Kaech and Gary Banker Published online: 11 January 2007 | doi:10.1038/nprot.2006.356 Abstract | Full Text | PDF (544K)
Extraction and analysis of soluble inositol polyphosphates from yeastpp2416 - 2422
Cristina Azevedo and Adolfo Saiardi Published online: 21 December 2006 | doi:10.1038/nprot.2006.337 Abstract | Full Text | PDF (365K)
A fluorescence-based protocol for quantifying angiotensin-converting enzyme activitypp2423 - 2427
Miguel Angel Sentandreu and Fidel Toldra Published online: 29 December 2006 | doi:10.1038/nprot.2006.349 Abstract | Full Text | PDF (151K)
Multiplex-terminal restriction fragment length polymorphismpp2428 - 2433
Brajesh K Singh and Nadine Thomas Published online: 29 December 2006 | doi:10.1038/nprot.2006.392 Abstract | Full Text | PDF (232K)
Triflimide (HNTf2)-catalyzed aldehyde cross-aldol reaction using [ldquo]super silyl[rdquo] enol etherspp2434 - 2438
Matthew B Boxer and Hisashi Yamamoto Published online: 29 December 2006 | doi:10.1038/nprot.2006.389 Abstract | Full Text | PDF (210K)
Identification of eukaryotic secreted and cell surface proteins using the yeast secretion trap screenpp2439 - 2447
Sang-Jik Lee, Byung-Dong Kim and Jocelyn K C Rose Published online: 29 December 2006 | doi:10.1038/nprot.2006.373 Abstract | Full Text | PDF (290K)
Measuring the optokinetic response of zebrafish larvaepp2448 - 2451
Susan E Brockerhoff Published online: 29 December 2006 | doi:10.1038/nprot.2006.255 Abstract | Full Text | PDF (176K)
Staining protocol for organotypic hippocampal slice culturespp2452 - 2456
Nadine Gogolla, Ivan Galimberti, Vincenzo DePaola and Pico Caroni Published online: 29 December 2006 | doi:10.1038/nprot.2006.180 Abstract | Full Text | PDF (279K)
High-throughput knockout screen in fission yeastpp2457 - 2464
Juraj Gregan et al. Published online: 29 December 2006 | doi:10.1038/nprot.2006.385 Abstract | Full Text | PDF (737K)
A protocol for TILLING and Ecotilling in plants and animalspp2465 - 2477
Bradley J Till, Troy Zerr, Luca Comai and Steven Henikoff Published online: 29 December 2006 | doi:10.1038/nprot.2006.329 Abstract | Full Text | PDF (484K)
IRAP and REMAP for retrotransposon-based genotyping and fingerprintingpp2478 - 2484
Ruslan Kalendar and Alan H Schulman Published online: 11 January 2007 | doi:10.1038/nprot.2006.377 Abstract | Full Text | PDF (305K)
One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalitiespp2485 - 2492
Hai Yu, Harshal A. Chokhawala, Shengshu Huang and Xi Chen Published online: 11 January 2007 | doi:10.1038/nprot.2006.401 Abstract | Full Text | PDF (295K)
Error-prone rolling circle amplification: the simplest random mutagenesis protocolpp2493 - 2497
Ryota Fujii, Motomitsu Kitaoka and Kiyoshi Hayashi Published online: 29 December 2006 | doi:10.1038/nprot.2006.403 Abstract | Full Text | PDF (265K)
A peroxidase-coupled continuous absorbance plate-reader assay for flavin monoamine oxidases, copper-containing amine oxidases and related enzymespp2498 - 2505
Andrew Holt and Monica M Palcic Published online: 29 December 2006 | doi:10.1038/nprot.2006.402 Abstract | Full Text | PDF (245K)
Immunofluorescence microscopy and immunogold EM for investigating fungal infection of plantspp2506 - 2511
Christopher R Thornton and Nicholas J Talbot Published online: 29 December 2006 | doi:10.1038/nprot.2006.347 Abstract | Full Text | PDF (302K)
Collagen antibody-induced arthritispp2512 - 2516
Levon M Khachigian Published online: 29 December 2006 | doi:10.1038/nprot.2006.393 Abstract | Full Text | PDF (285K)
Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue techniquepp2517 - 2526
Aysha H Osmani, Berl R Oakley and Stephen A Osmani Published online: 29 December 2006 | doi:10.1038/nprot.2006.406 Abstract | Full Text | PDF (363K)
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