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J Ethnopharmacol. 2005 Jan 4;96(1-2):287-94.
Tan ML, Sulaiman SF, Najimuddin N, Samian MR, Muhammad TS.
School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Currently, breast cancer is the leading cause of cancer-related death in women. Therefore, there is an urgent need to develop alternative therapeutic measures against this deadly disease. Here, we report the cytotoxicity activity and the mechanism of cell death exhibited by the methanol extract prepared from Pereskia bleo (Kunth) DC. (Cactaceae) plant against human breast carcinoma cell line, T-47D. In vitro cytotoxicity screening of methanol extract of Pereskia bleo plant indicated the presence of cytotoxicity activity of the extract against T-47D cells with EC50 of 2.0 microg/ml. T-47D cell death elicited by the extract was found to be apoptotic in nature based a clear indication of DNA fragmentation which is a hallmark of apoptosis. In addition, ultrastructural analysis also revealed apoptotic characteristics (the presence of chromatin margination and apoptotic bodies) in the extract-treated cells. RT-PCR analysis showed the mRNA expression levels of c-myc, and caspase 3 were markedly increased in the cells treated with the plant extract. However, p53 expression was only slightly increased as compared to caspase 3 and c-myc. Thus, the results from this study strongly suggest that the methanol extract of Pereskia bleo may contain bioactive compound(s) that caused breast carcinoma, T-47D cell death by apoptosis mechanism via the activation of caspase-3 and c-myc pathways.
Peireskia bleo in Martius's 'Flora Brasiliensis'
Recommended Temperature Zone:|
Minimum Avg. Temperature: 50°F (10°C)
Heat Tolerance: Light shade in Phoenix in summer
Sun Exposure: Morning sun or bright shade
Origin: Panama and Colombia
Watering Needs: Enjoy some humidity in the air. They need good drainage, but the soil should stay moist in summer. Keep drier in winter.