[WORK OS Plus 40 Cubo Sistemas Serial
Gildo Santiago
The plug-and-play QIAcube Connect simplifies purification by automating QIAGEN spin-column kits. No change of chemistry is needed, so startup is fast, results are immediate, and performance is comparable to the manual procedure.
QIAcube Connect includes a centrifuge, heated shaker, pipetting system and robotic gripper (see " The QIAcube Connect worktable"). These let the QIAcube Connect fully automate more than 80 QIAGEN spin column kits.
QIAcube Connect decontaminates your full worktable with built-in UV light (see " The QIAcube Connect UV light") to prevent carryover between samples (see " Genomic DNA (gDNA) barely detectable after UV light exposure").
Stay alert to important instrument events with QIAsphere's push notifications. You can even check maintenance status and manage run reports from anywhere, at anytime. Increase the efficiency and productivity of your lab. Learn more about QIAsphere.
QIAcube Connect has a variety of pre-installed protocols for purification of RNA, genomic DNA, plasmid DNA, viral nucleic acids and proteins, plus DNA and RNA cleanup. All standard protocols in the expanding range can also be downloaded free of charge. Customized protocols can also be requested.
A large touchscreen (see figure '' The QIAcube Connect touchscreen'') simplifies protocol selection. Clear on-screen messages guide you through worktable setup. You are unlikely to make mistakes because the labware and accessories fit onto the worktable only in the correct orientation. In addition, a fully automated load check helps to ensure that samples, reagents and labware are loaded correctly.
Dedicated QIAcube Connect kits further simplify automated spin-preps for purification of RNA, genomic DNA and viral RNA. Rotor-adapters that come with the kits are pre-loaded with spin columns and elution tubes, so they are ready to use right out of the box. And then, to lessen the risk of errors even more, tubes required for the manual procedure are not included in kits dedicated for use with QIAcube Connect.
QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.
Yes. The heated shaker on the QIAcube Connect can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the shaker, refer to the QIAcube Connect User Manual, chapter 5 "Operating Procedures", section 5.9 "Independent heater/shaker operation".
Use the USB flash drive that was shipped with the QIAcube Connect to transfer the protocol files to the instrument. Create a new folder on the USB flash drive with the name "Protocol_Upload" and copy the new protocol zip file(s) to this directory. Do not unzip the files. Make sure to use the correct directory; otherwise, the QIAcube Connect will not find the protocols.
Note: Do not rename or modify the protocol files. Otherwise, they cannot be used.
All protocol zip file(s) in the "Protocol_Upload" folder will be installed.
Note: Already installed protocols will not be overwritten. If you attempt to re-install an existing protocol, a message will appear indicating that not all protocols could be copied.
Yes. At the end of each protocol run on the QIAcube, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.
Report files can be automatically transferred from your QIAcube Connect using the QIAsphere Export Tool. For this, just connect your QIAcube Connect to QIAsphere. More information can be found on the QIAsphere webpage.
You can stop a QIAcube protocol if there is an emergency by pressing Stop Run. To confirm that you want to stop the protocol run, press Yes. To cancel the "Stop Run" command, press No.
Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed on the touchscreen. For more information, refer to the QIAcube Connect User Manual.
Yes, this is absolutely necessary. If the lids are not securely fixed in the rotor adapter slots during processing on the QIAcube, they will become detached from the elution tubes and columns during centrifugation and will collect at the bottom of the centrifuge chamber.
No. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. Do not use spin columns manufactured by other suppliers with the QIAcube. Damage caused by use of other types of spin columns will void the instrument warranty.
Protocols for automated DNA extraction from saliva stabilized with the PAXgene Saliva Collector are available for the QIAsymphony DNA Midi Kit with the QIAsymphony SP instrument or the QIAamp DNA Mini Kit with the QIAcube (Classic and Connect).
For manual extraction, supplementary protocols are available for the QIAamp DNA Mini and Gentra Puregene Cell Kits (see resources section).
No. Slightly modified QIAGEN protocols or protocols fully customised to your specific requirements can be requested from QIAGEN. Our dedicated team of specialists has already customised over 5000 protocols.
Dedicated QIAcube kits are the RNeasy Mini QIAcube Kit, QIAamp DNA Mini QIAcube Kit, the QIAamp DNA Blood Mini QIAcube Kit, and the QIAamp Viral RNA Mini QIAcube Kit. In addition to these kits are the QIAamp PowerFecal Pro DNA QIAcube Kit, the DNeasy PowerSoil Pro QIAcube Kit using Power technology, and the DNeasy Blood & Tissue QIAcube Kit.
Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed in the touch screen. For more information, refer to the QIAcube User Manual.
El paquete de iluminacin Premium reemplaza todos los faros anteriores halgenos y de descarga de alta intensidad (HID) por luces LED. Las nicas luces que no son LED son los faros de conduccin de luz corta; estos siguen siendo halgenos. Esto permite que cada luz LED funcione a una temperatura ms baja y que ninguna luz trabaje ms que otra. La uniformidad de la cobertura LED permite un solo tipo de salida de luz alrededor del tractor.
En el paquete Premium, la distribucin de la iluminacin brinda una visibilidad nocturna con el mejor desempeo en la industria. Las bombillas LED proporcionan un brillo mximo y una salida de color autntico para una excelente definicin de campo que ayuda a los ojos del operador.
Estas luces LED proporcionan un 40 por ciento ms de ancho de cobertura y un 10 por ciento ms de cobertura de luz en la parte trasera. Los paquetes de iluminacin LED usan un 45 por ciento menos de amperios que las luces estndar halgenas, y tienen una vida til de ms de 10000 horas, lo cual reduce los costos de propiedad sobre la vida til del tractor.
NOTA: Los paquetes de iluminacin pueden variar segn las zonas.
NOTA: Las luces de carretera/cargadora tambin se conocen como luces de conduccin altas para aplicaciones del enganche delantero que ensombrecen a los faros de carretera. Consultar la seccin Accesorios.
La funcin de ahorro de batera tambin es estndar. Cuando el motor est apagado y las luces exteriores se han dejado encendidas, esta funcin evita que la batera se gaste.
Si las luces se han dejado encendidas durante 30 minutos y la llave est en posicin de desconexin, las luces parpadearn cinco veces a modo de advertencia. Las luces seguirn encendidas durante un minuto ms y luego se apagarn automticamente para proteger la batera.
Otra caracterstica es la iluminacin de salida programable. La iluminacin de salida permite que las luces en el exterior de la cabina permanezcan encendidas hasta por 300 segundos. Pueden programarse en incrementos de 0 a 300 segundos.
Consultar el manual del operador para ver las instrucciones completas para programacin en el campo, faros de carretera e iluminacin de salida.
Gracias al sistema ILS se consigue una mayor transmisin de potencia al suelo dado que los neumticos delanteros mantienen la presin de contacto con el mismo. De este modo se mejora el desplazamiento en campo y el transporte a la vez que se aumenta la flexibilidad de lastrado y se reduce significativamente la tendencia a producirse saltos de potencia.
El sistema de suspensin mecnica est compuesto por dos grandes brazos de control superior e inferior de hierro fundido con manguetas de direccin tambin de hierro fundido para garantizar la durabilidad y la fiabilidad.
Los sistemas mecnico, hidrulico y elctrico trabajan conjuntamente para mantener una posicin nivelada y verticalmente centrada del crter del diferencial delantero en relacin con los cubos y planetarios exteriores, independientemente del peso del tractor o de la carga dinmica. La capacidad del sistema de mantener una posicin verticalmente centrada posibilita un desplazamiento de la suspensin completa de 25,4 cm (10 pulgadas). Esto se traduce en un contacto homogneo con el suelo para lograr una mayor transmisin de potencia al suelo, adems de una amortiguacin de la energa producida por las sacudidas que ocasionan una conduccin inestable.
El tractor utiliza controles electrnicos e informticos para supervisar las funciones del mismo as como la posicin del eje. En funcin de estos datos, el sistema elctrico activa de forma automtica las funciones hidrulicas para elevar, bajar o permanecer esttico.
La suspensin independiente de John Deere emplea un diseo de brazo A corto/largo, lo cual significa que los brazos de control superiores son ms cortos que los inferiores. Durante el movimiento de las ruedas, el brazo de control superior se desplaza en un arco ms reducido que el inferior y la diferencia entre ambos arcos da lugar a una modificacin de la cmara. Dicha modificacin contribuye a mantener el interior y el exterior de los neumticos delanteros perpendiculares a la superficie de conduccin (una funcin de vital importancia cuando estn instalados neumticos delanteros duales opcionales). Este diseo presenta funciones antihundimiento para reducir de forma natural los efectos adversos del frenado y la aceleracin.
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