I am trying to extract the Coverage track data including total count, breakdown of nucleotides, and forward/reverse read breakdown from the IGV interface.
This information appears in a pop up box when you hover over a column in the coverage track.
I am using v2.4.10 of the interface on a Windows 10 OS. I have not used the command line version but i am not adverse to doing so if this would help.
I have tried using the count command in IGVtools with a window size of one. This gives me a .wig file with the total count information per bp with out any of the breakdown.
I have also tried using both samtools and bcftools with no success but any suggestions would be very helpful.
Is there another way to export this information into a table? Is it also possible to take a larger file and export select ranges of the coverage information by referencing a .bed file?
Thank you,
-John Spainhour