I have PacBio HiFi reads with MM and ML tags indicating base modifications. When I open the BAM file in IGV and set
Color alignments by → Base modification → 2-color (5mC), all modified bases appear blue (as shown in image 1).

Upon closer inspection, I noticed that the coloring behavior differs depending on whether I select
Base modification (5mC) or
2-color (5mC). In the 2-color mode, bases with base modification:5mC 74% were colored in dark
blue not red. (images 2 and 3)).

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I've also attached my
Color Legend settings (image 4) and
Preferences → Alignment Track Default settings (image 5) for reference.
.


My goal is to clearly distinguish methylated and unmethylated bases using
red and blue coloring.
What settings or adjustments can I make in IGV to achieve this?