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Venura Herath PhD

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Jun 20, 2019, 3:26:04 PM6/20/19
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Hi,

I have created .genome file for Nicotiana benthamina genome downloaded from ftp://ftp.solgenomics.net/genomes/Nicotiana_benthamiana/. I also downloaded GFF3 file with all annotations.
I tried two ways to get my annotations to appear but both failed.
1. when creating the genome file I included gff files with gene models. After loading i couldn't see gene models. Then i tried to load file as a track using load file. Result was the same where i see a track but not data . Then i sorted and indexed the file. When i load indexed file again no annotations were visible.
2. Then I tried to do the same process with GFF3 file with all annotations. No sucess.
Can you please help me on this regard.
Thank you,
Venura

James Robinson

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Jun 20, 2019, 5:26:24 PM6/20/19
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Could you check that the sequence names in the GFF3 file are the same as the sequence names in your fasta?

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