Codex Steam Emu

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This product falls under the category of antifungal preservatives (see potassium sorbate). Because it is not soluble in wine, it cannot be used as is, but rather in its potassium salt form. It may be soluble in some spirits.

Shake 0.5 g of sorbic acid with 70 ml of boiling water. Let the solution cool. Filter and collect the filtrate in a 100 ml volumetric flask. Wash the first container and the precipitate and the filter several times with several ml of water until 100 ml of filtrate is obtained.

To 20 ml of solution prepared for tests under paragraph 5.3, add 1 ml hydrochloric acid diluted to 10 pp 100 (R) and 2 ml of barium chloride solution (R). The mixture should be clear, or the opalescence observed after 15 minutes should be less than that of the control solution prepared as indicated in the Annex. (Sulfate content expressed in terms of sulfuric acid should be less than 1 g/kg).

To 10 ml of solution prepared for tests under paragraph 5.3, add 5 ml of water, 5 ml of nitric acid diluted to 10 pp 100 (R) and 0.5 ml of 5 pp 100 silver nitrate solution (R). The mixture should be clear or the opalescence observed after 15 minutes should be less than that of the control solution prepared as indicated in the Annex. (Chloride content expressed in terms of hydrochloric acid should be less than 1 g/kg).

Take 10 ml of the solution prepared under paragraph 5.3. Add 2 ml of pH 3.5 (R) buffer solution, and 1.2 ml of thioacetamide reagent (R). Use the method described in the Annex. (Heavy metal content expressed in terms of lead should be less than 10 mg/kg).

1 Weigh a quantity p of sorbic acid of about 0.20 g and dissolve it in 10 ml of pure alcohol. Then dilute in 100 ml of water. Titrate the acidity using a 0.1M solution of sodium hydroxide in the presence of phenolphthalein solution (R). Let n be the amount in ml used: 1 ml of 0.1M sodium hydroxide solution corresponds to 0.0112 g of sorbic acid. Content in pp 100 of sorbic acid in the product tested:

2 The same procedure should be performed after entrainment in steam. Place 10 ml of the alcoholic solution containing a quantity of p grams of sorbic acid (about 0.2 g) in the bubble chamber of a steam distillation machine. Add a crystal (about 0.5 g) of tartaric acid and distill at least 250 ml (until the steam no longer distills acid). Titrate the distilled acidity using a 0.1M sodium hydroxide solution.

The IUPAC method II.D.27 for detection of reesterified oils by analysis of fatty acids at position-2 in fat and oil glyceride is reinvestigated and the effect of storage and different refining processes on the glyceride structure of genuine olive oil and olive-residue oil is studied. The method is unsuitable for oils neutralized by steam distillation because of changes in the triglyceride structure. An improved procedure is proposed in order to minimize erroneous conclusions for several other analyses.

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