bug with substract or merge ROI-initialization of ROI

[IcyLyd]

Hi guys,
I'm trying to substract two ROI in one picture and to run it successively on several pictures.
I ran a protocole in batch (several picture in a folder loop) with substract ROI blocks.
When one of my ROI is absent (actually black because nothing thresholded for some pictures), instead of doing the substraction minus "nothing" the substraction is done with a previous ROI (I mean the one from image n-1 instead of the empty ROI of image n).  Is it normal? Is it possible to intilialize all the ROI between two iteration of a folder loop protocole to avoid that bug ?
Thanks.
Lydia

[Alexandre Dufour]

Hi Lydia,

Thanks for reporting this. This bug is now fixed for all 3 blocks (merge, subtract and translate).

Have fun!
Alexandre

[IcyLyd]

Thank you so much, it works now. And my big SIM Colocalisation protocole is running wonderfully !
:)