Problems with merge channels

ethanwan...@gmail.com

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Oct 31, 2018, 5:58:58 AM10/31/18

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Dear all,

I did some Fluorescence-based Cell Microscopequantifications of colocalization. The problem is that if I try to merge different channels into one picture (same cells, with different bio-markers), I don't get the right overlap. (I include a few pictures to show you an example)

Can someone help me how to solve it?

Thanks a lot for any kind of help you can bring

Best,

Ethan

Problem with merge channels 2.png

Problem with merge channels 1.png

Stephane

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Nov 6, 2018, 5:51:48 AM11/6/18

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Hi Ethan,

You can use the Rigid Registration plugin (just type "Registration" in the Icy top search bar) to correct the channel drift in your merged image, that should make it :)

Best,

- Stephane

ethanwang0072018

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Nov 6, 2018, 9:37:58 PM11/6/18

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Hi Stephane,

Thanks so much for your help. You are very very kind! Your advice gave me a great help for my data processing.

I really appreciate it.

Best,

Ethan

发自网易邮箱大师

On 11/6/2018 18:51，Stephane<stephane.da...@pasteur.fr> wrote：

Hi Ethan,

You can use the Rigid Registration plugin (just type "Registration" in the Icy top search bar) to correct the channel drift, that should make it :)

Best,

- Stephane

Le mercredi 31 octobre 2018 10:58:58 UTC+1, ethanwang0072018 a écrit :
Dear all,

I did some Fluorescence-based Cell Microscopequantifications of colocalization. The problem is that if I try to merge different channels into one picture (same cells, with different bio-markers), I don't get the right overlap. (I include a few pictures to show you an example)

Can someone help me how to solve it?

Thanks a lot for any kind of help you can bring

Best,

Ethan

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