Image format change when copying

Masoud Ramuz

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Jun 22, 2018, 10:24:36 AM6/22/18

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Dear Stephane,

Sorry to bother you again, but I'm having a very troublesome problem.

When imaging using Micro-Manager the images seem to be opened in TIF format (I can't confirm but my pixel intensities can go up to over 5000) however when I copy an image made by Micro-Manager using either the IcyBufferedImage.copyData method or the screenshot button on the viewer, I get maximum pixel intensities of 255. I believe this indicates that the image is converted to a PNG?

This makes comparing intensities between images more difficult, but most importantly the loss of resolution going from an intensity range of 0 to over 5000 (not sure what the limit of MM images is for this), to 0-255 is troubling.

Any ideas how I could solve this issue and keep the same format of images when copying them?

Thank you very much,

Masoud Ramuz

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Jun 22, 2018, 10:29:23 AM6/22/18

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To illustrate my point:

Auto Generated Inline Image 1

Masoud Ramuz

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Jun 22, 2018, 11:31:19 AM6/22/18

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Apologies, after further testing the copyData method doesn't seem to be the culprit. The screenshot button however still does this.

On Friday, June 22, 2018 at 3:24:36 PM UTC+1, Masoud Ramuz wrote:

Stephane

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Jun 25, 2018, 5:03:01 AM6/25/18

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Hi Masoud,

The screen shot does it, that is expected as screen shot button just convert / flatten the image to a classic 8bit RGB image so you can save it to PNG / JPG easily :)

What do you want to do here ? you can use crop command to crop and maintain the original format.