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Eric
Jul 17, 2017, 12:18:31 PM7/17/17
to Icy imaging
Hi all,
I am a bit sneaky but I tried to verify if my settings were correct to use the colocalization studio on objects
When I try to compare the coloc on the same image the colocalized spots are at 0 and Ripley is negative as if the plugin did not work.
If I substract an intensity of 10 to one of the image (using imageJ). then I obtain good results with a high Ripley. See enclose images to try and screen capture.
Thanks for your comments
I am a bit sneaky but I tried to verify if my settings were correct to use the colocalization studio on objects
When I try to compare the coloc on the same image the colocalized spots are at 0 and Ripley is negative as if the plugin did not work.
If I substract an intensity of 10 to one of the image (using imageJ). then I obtain good results with a high Ripley. See enclose images to try and screen capture.
Thanks for your comments
Thibault Lagache
Jul 27, 2017, 10:42:25 PM7/27/17
to Icy imaging
Hi Eric,
Sorry for the late answer. I think there is a problem with the object based methods when the distance between spots'localizations is exactly equal to 0. I think that when you decrease the image intensity, it changes very slightly spots'coordinates and leads to a positive measured coupling. I will fix this issue as soon as possible.
Thanks
Thibault Lagache
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Eric
Jul 28, 2017, 5:40:17 AM7/28/17
to Icy imaging
Thanks Thibault for your answer,
I wish you will find a fix for this problem !!!
I tried the coloc by moving the second image by two pixel and it worked perfectly !!!
I feel better now !!!
Thanks again
I wish you will find a fix for this problem !!!
I tried the coloc by moving the second image by two pixel and it worked perfectly !!!
I feel better now !!!
Thanks again
Le lundi 17 juillet 2017 18:18:31 UTC+2, Eric a écrit :
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