Subtract ROI

[Mike Kress]

Dear Developers,

I am using the following  protocol : “Find cells using nuclear and membrane fluorescence”, ROI of nuclei is obtained using block “HK-means” (HK-means detection) and ROI corresponding to cytoplasm is obtained using block “Active contours” (ROI contour (Area 2D)). My goal is to detect objects present only in cytoplasm, so I subtracted ROI nuclei minus ROI cytoplasm and I got an error message: “Can't process subtraction: ROI 1 has infinite C dimension while ROI 2 has a finite one”

Could you explain me this error message and how to only get ROI corresponding to cytoplasm?

Thank you very much in advance.
Sincerely,

Michel

[Stephane]

Hi Mike,

Logic operation between ROI can work only for ROI with comparable dimension.
What you can do is to set the C position of the ROIs to "infinite" before doing the operation, for that you need to use the block called "Set ROI position C" and set 'position C' parameter to -1 (-1 = infinite here).
Hope that will help you :)

Best,

- Stephane

[Stephane]

Oh i forgot, the protocol block called "Set ROI position C" is bundled in the ROI blocks plugin (maybe you will need to install / update it if you don't find the block).

[Mike Kress]

Hi Stéphane,
Thank you for your answer.
It works.
Michel

Le jeudi 12 janvier 2017 11:36:43 UTC+1, Mike Kress a écrit :