Vicki,
I am in Vancouver BC. Where I have only seen one native monarch in my life. I get mine shipped in from the East and breed them to get livestock. I only breed them for several generations, mixing new stock in to ensure no inbreeding effects take hold. Then the resulting livestock is released. I manage to raise hundreds from just a single pair of monarchs to begin with.
This may be your only hope when it comes to preventing disease. Especially those that seem to spring up from nothing. All your practices seem to be perfect so there are no alarms there. I would have a breeding stock going so all your efforts are not wasted. You can control everything from the moment that egg is laid (all the eggs we collect are from indoor lab conditions, on cut milkweed).
I usually have monarchs overwintering in the fridge but my stock last year did do so well towards the end of the season so they were set free.
I would start fresh, sanitize everything with a 70% solution of alcohol (not 99% because it is actually less strong) and test everything for Oe spores and bacterial outbreaks. Any compound microscope on eBay will do.
I let the lab mellow for several months on the off season so nothing can establish itself within. In the spring everything gets sanitized again even if I already did a before the mellow period and livestock is brought in.
I never ever ever, especially for Painted Ladies, bring in new livestock if my existing culture is bright and healthy. This I've learned the hard way as potential for disease to be brought in increases hundredfold if you are not careful. I would only recommend this process to yourself, a person who dedicates hard work into raising these important insects.
It is very likely Oe has seen your set up. All it takes is an infected female to leave behind spores when the egg is laid to waste you 4 weeks until one day the caterpillars stop eating and eventually don't make it. That is why you hear breeders stressing so much about bleaching the eggs and taking them off original leaves. Interestingly though, tropical milkweed will allow you to raise a majority of these infected larvae to HUGE sizes and emerge into LARGE adults when other types of milkweed result in fast infection and death. These adults as always, have to be euthanized.
Sorry for such a long email. Please let me know if this helps. I raise many other species as well, a benefit we individuals get from living in the northern regions so don't hesitate if you want to try other types of Lepidoptera but have questions.
All the best,
Josh