I used the humann2 tool previously for my clinical-based data analyses. However, when I wanted to use it again a few months later, it did not work anymore. Consequently, I re-installed humann2 and the required databases but I still get the following error message, when I try to run humann2:
Decompressing gzipped file ...Running metaphlan2.py ........Traceback (most recent call last): File "/miniconda3/bin/humann2", line 11, in <module> sys.exit(main()) File "/miniconda3/lib/python2.7/site-packages/humann2/humann2.py", line 906, in main custom_database = prescreen.create_custom_database(config.nucleotide_database, bug_file) File "/miniconda3/lib/python2.7/site-packages/humann2/search/prescreen.py", line 93, in create_custom_database read_percent=float(line.split("\t"))ValueError: invalid literal for float(): 2|1239|91061|186826|1300|1301|2803
Do you have a bit of advice on what I can do about it?
Thank you very, very much in advance!