HLAthena server returns error

[Laurenz...@ugent.be]

Dear Sarkizova S.,

Congratulations on the development of HLAthena.

I was trying to run your algorithm through the HLAthena server, when I repeatedly got the following error message: 'An error has occurred. Check your logs or contact the app author for clarification.'. As this was not much to go on I tried to remove peptides that potentially were too long as well as test whether amino acids residues such as X or U might be problem. Neither appeared to give this same error message. Making me wonder whether the size of the input data might be a problem, which was around 5000-6000 peptides. However, according to the information on the site this should be okay.

Could you point me in the right direction to solve this error?

Kind regards,

Laurenz De Cock

[Sisi Sarkizova]

Hi Laurenz, 

Would you be willing to share the list of peptides and alleles you are predicting for (and any other input parameters you are specifying) so that I can try to reproduce the problem and debug on my end?

Thanks,

Sisi

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[Sisi Sarkizova]

I know you mentioned that you made sure there were no non-amino-acid symbols in the peptides but I was wondering if your input contained upstream and downstream sequences. If so, one thing worth double checking would be that there are no such symbols in those sequences as well?

Sisi

[Laurenz...@ugent.be]

Dear S. Sarkizova,

I checked the peptide, up- and downstream sequences for non-amino-acid symbols (X, U and Z) and after removal of these HLAthena ran flawlessly this morning. I must have overlooked this, my apologies.

Kind regards,

Laurenz De Cock

On Wednesday, February 12, 2020 at 1:32:00 AM UTC+1, Sisi Sarkizova wrote:

I know you mentioned that you made sure there were no non-amino-acid symbols in the peptides but I was wondering if your input contained upstream and downstream sequences. If so, one thing worth double checking would be that there are no such symbols in those sequences as well?

Sisi

On Tue, Feb 11, 2020 at 1:37 PM Sisi Sarkizova <sisi.s...@gmail.com> wrote:

Hi Laurenz, 

Would you be willing to share the list of peptides and alleles you are predicting for (and any other input parameters you are specifying) so that I can try to reproduce the problem and debug on my end?

Thanks,

Sisi

On Tue, Feb 11, 2020 at 11:35 AM <Lauren...@ugent.be> wrote:

Dear Sarkizova S.,

Congratulations on the development of HLAthena.

I was trying to run your algorithm through the HLAthena server, when I repeatedly got the following error message: 'An error has occurred. Check your logs or contact the app author for clarification.'. As this was not much to go on I tried to remove peptides that potentially were too long as well as test whether amino acids residues such as X or U might be problem. Neither appeared to give this same error message. Making me wonder whether the size of the input data might be a problem, which was around 5000-6000 peptides. However, according to the information on the site this should be okay.

Could you point me in the right direction to solve this error?

Kind regards,

Laurenz De Cock

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[Sisi Sarkizova]

Glad it worked well!

Sisi

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