Faster bowtie2 parameters

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Ricardo Linares

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Feb 5, 2025, 12:03:17 AMFeb 5
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HiC-Pro has the following default parameters for mapping:
 `BOWTIE2_GLOBAL_OPTIONS = --very-sensitive -L 30 --score-min L,-0.6,-0.2 --end-to-end --reorder
BOWTIE2_LOCAL_OPTIONS =  --very-sensitive -L 20 --score-min L,-0.6,-0.2 --end-to-end --reorder`

These are very stringent, and often takes a long time (also there is not explanation why the use of a custom seed length). I was wondering if anyone has used and/or recommends different parameters that are faster without compromising sensitivity too much? maybe changing the --very-sensitive to --sensitive or --fast is a way to achieve this?
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