Was there a reason you attempted to set the bias? The bias is preset at the factory. Now that you have adjusted the bias, is 166mA the maximum you can get, not the recommended ~205mA?
To eliminate the PA, can you disable the PA then measure and report output from the low power RF1 output?
Quisk will sometimes not transmit if the audio card settings are missing are incorrect. Can you please post a screenshot of your Quisk audio setup? Also, please try with sparkSDR to help eliminate software configuration.