Therevised edition of the bestselling textbook, covering both classical and molecular plant breeding
Principles of Plant Genetics and Breeding integrates theory and practice to provide an insightful examination of the fundamental principles and advanced techniques of modern plant breeding. Combining both classical and molecular tools, this comprehensive textbook describes the multidisciplinary strategies used to produce new varieties of crops and plants, particularly in response to the increasing demands to of growing populations. Illustrated chapters cover a wide range of topics, including plant reproductive systems, germplasm for breeding, molecular breeding, the common objectives of plant breeders, marketing and societal issues, and more.
Now in its third edition, this essential textbook contains extensively revised content that reflects recent advances and current practices. Substantial updates have been made to its molecular genetics and breeding sections, including discussions of new breeding techniques such as zinc finger nuclease, oligonucleotide directed mutagenesis, RNA-dependent DNA methylation, reverse breeding, genome editing, and others. A new table enables efficient comparison of an expanded list of molecular markers, including Allozyme, RFLPs, RAPD, SSR, ISSR, DAMD, AFLP, SNPs and ESTs. Also, new and updated "Industry Highlights" sections provide examples of the practical application of plant breeding methods to real-world problems. This new edition:
* Organizes topics to reflect the stages of an actual breeding project
* Incorporates the most recent technologies in the field, such as CRSPR genome edition and grafting on GM stock
* Includes numerous illustrations and end-of-chapter self-assessment questions, key references, suggested readings, and links to relevant websites
* Features a companion website containing additional artwork and instructor resources
Principles of Plant Genetics and Breeding offers researchers and professionals an invaluable resource and remains the ideal textbook for advanced undergraduates and graduates in plant science, particularly those studying plant breeding, biotechnology, and genetics.
Stewart teaches courses in biotechnology and research ethics. He has mentored over 100 graduate and postdoctoral students and technical staff, most of whom are still in science. Stewart has given scientific and lay presentations around the US and in 16 countries.
This project focuses on having a validated and reliable long-distance transport prediction model for wind-dispersed pollen is critical to establishing appropriate isolation distances for genetically engineered crops and making informed regulatory decisions. Switchgrass and hemp are primarily wind-pollinated crops. The specific objectives of our proposed research are to develop and test new methods with unmanned aircraft systems (UAS or drones) for monitoring wind-dispersed pollen from genetically engineered crops, generate and validate a large eddy simulation (LES) model to track the long-distance transport of genetically engineered pollen, and predict the regional transport of wind-dispersed pollen using the LES model. Genetically engineered switchgrass and hemp, in which pollen is marked with genetically-encoded fluorescent proteins, will be grown at a unique field site in Tennessee. This work is being done in collaboration with Assistant Professor Hosein Foroutan, Professor Shane Ross, and Professor David Schmale laboratories at Virginia Tech.
This project focuses on increasing the yield and sustainability of plant feedstocks, which emphasizes the importance of performing field studies for improvement of biofuel yield without negative consequences to biomass production or sustainability. Our study supports a genome-wide association study (GWAS) in switchgrass under field condition to enable rapid domestication and increased sustainability in feedstock. The study also involves the development of unmanned aerial vehicle (UAV)-based remote sensing for switchgrass high-throughput phenotyping in the field. The UAV-based approach facilitates identification of superior genotypes. Using a GWAS approach, many measurements, including growth, composition and biomass convertibility, of the broad variation across the switchgrass population can be correlated with specific genes. The use of the natural variation and identification tools in the GWAS switchgrass studies allow rapid identification of gene candidates. Our established GWAS field enables rapid discovery of environmentally stable genetic controls. This will lead to the identification of important genetic regulators of biomass recalcitrance and other plant traits bringing the increased yield and sustainability in long-lived perennial switchgrass.
This project focuses on soybean plants with increased root length and branching to boost yield and durability. A strong root system is necessary for maximized plant nutrition, defense, and water use. Soybean plants with increased root length and branching would significantly improve water and nutrient uptake efficiency and yield potential in diverse environments. Such improvement is the primary goal of this project. For that, the key soybean genes involved in root growth and development are identified. The candidate root growth genes are initially assessed in transgenic soybean hairy root system. Subsequently, the novel root genes are evaluated in genetically-modified stable soybean plants for their impact on root growth, soybean productivity, and stress tolerance to challenging environmental conditions. We expect that the discovery of the novel root-important genes identified in the present study will lead to the production of soybean with deeper and more extensive root systems. In turn, these plants will have improved performance and yield, particularly under conditions of limited water and nutrient availability. The present study provides a basis for development of soybean lines with improved growth and tolerance to abiotic stresses. The result should facilitate a roadmap for breeding and developing high yielding soybean varieties.
The project focuses on producing genetically engineered soybean for deer resistance traits. All legume seeds produce trypsin inhibitors as well as other protease inhibitors that can hinder mammalian digestive enzymes. Mature seeds must be treated by heat or chemicals to deactivate these protease inhibitor proteins that are found in soybean meals. Soybean, therefore, already has a built-in deterrent to feeding by mammals in the seed. The goal of this project is to produce trypsin inhibitors in soybean leaves with high expression. For that, 6 different Kunitz trypsin inhibitor genes and 1 Bowman-Birk inhibitor gene have been selected. These genes will be evaluated in genetically engineered soybean plants to determine their inhibitory impact on the mammalian digestive system. Since deer are adept learners, it is hypothesized they would learn to avoid eating soybean leaves. Additionally, Kunitz trypsin inhibitor inactivates trypsin enzyme, which is the common digestive enzyme found in lepidopteran insects. The transgenic soybean lines will also be evaluated against leaf-feeding insects to determine their impact on insect growth as well as the decrease of leaf defoliation. The present study will alter trypsin inhibitor expression in soybean leaves with an increased inhibitory effect on mammalian and leaf defoliator digestive systems.
A course on genetic principles and techniques used in plant modification and principles of molecular and transmission quantitative genetics as applied to plant biotechnology. Prerequisites include BIOL 111 and 112.
A work on the best practices in all the major areas of research management and practice that are common to scientific researchers, especially those in academia. Aimed towards the younger scientist, the book critically examines the key areas that continue to plague even experienced and well-meaning science professionals.
A greater biological understanding is essential to make sound management regulatory decisions when also taking into consideration the processes that happen in conventional plants. Monitoring, modelling, and mitigation are the three most closely related elements of gene flow. The book includes both scientific reviews and perspectives on gene flow and experimental case studies, including studies of gene flow in soybean and poplar. The authors present diverse views and research methodologies to understand transgene flow.
This unit provides an introduction to understanding the principles of inheritance and the analysis of variation in all organisms. Prokaryotic, Mendelian, population, quantitative and evolutionary genetics are considered in lectures and practical exercises. The interactive laboratory classes are also used to examine methods used in genetic research, and are designed to promote experience in practising these methods.
Students are able to (1) discover the theory and concepts underlying the inheritance of traits in prokaryotes and eukaryotes. Gain an appreciation of the social and historical context in which genetics has developed
100% Online Unit. NO campus face-to-face attendance is required to complete this unit. All study requirements are online only. Unit is asynchronous delivery, with NO requirement for students to participate online at specific times.
100% Online Unit. NO campus face-to-face attendance is required to complete this unit. All study requirements are online only. Unit includes some synchronous components, with a requirement for students to participate online at specific times.
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