Msi Center Coreliquid

[Octavis Marquez]

DescriptionThe UCSD Phenotyping Core provides behavioral and metabolic testing to assist researchers reach their goals. We provide testing, training, and rental of equipment for many testing paradigms on a recharge basis. All tests must be in an approved protocol prior to scheduling. All scheduling requests must be made a minimum of two business days in advance.

Description: The Animal Care Program Diagnostic Services Laboratory provides a broad spectrum of clinical pathology and morphologic pathology services for research support (e.g., safety/toxicology testing, individual animal model-related tests,p henotype analysis), as well as colony and individual animal health diagnostics and care. Prices for these services are determined on a cost recovery basis. The diagnostic laboratory is overseen by a veterinary pathologist, and staffed by experienced laboratory technicians. The large range of procedures and tests conducted in-house by the laboratory is complemented and expanded by use of established outside reference laboratories when necessary, as coordinated by the laboratory.

Description: The Animal Care Program has a qualified staff available that can provide a variety of technical services to your lab on a recharge basis. Services that can be provided include but are not limited to all types of injection (IP, IM, IV, SC), blood and tissue collection, gavage, x-ray, surgical procedures, anesthesia, surgical nursing, post-op care, etc. The ability for ACP staff to provide a particular service is based on many factors. The ACP has Animal Health Technicians available to assist laboratory staff with hands on training for minor technical procedures such as handling and restraint, blood and tissue collection, etc.

Description: The Biochemical Genetics and Metabolomics Lab performs metabolic screening, organic acid, amino acid, and acylcarnitine analysis for researchers and patients at UC San Diego and non-UC San Diego medical institutions.

Description: Core equipment includes Skyscan 1076 MicroComputed Tomography scanner for in vitro samples, at 9, 18, and 35um voxel size. Basic scanning includes scan time, axial views, and data. Additional options include advanced image processing for 2-D and 3-D visualization, morphometry, mineral density, fixed charge density.

Description: Core services include protein identification from gels or complex mixtures, multidimensional separations prior to nanoLC MSMS, protein quantitation by SILAC and iTRAQ, TMT, identification of post-translational modification including phosphorylation (phosphor-proteome), acetylation, and methylation. Core equipment includes ABSciex 5600 triple-TOF, and Thermo Fusion Lumos Orbitrap with ETD.

Description: CRMS performs CNC machining and design consultation to meet customers'' specialized needs. CAD/CAM technology enables us to machine complex parts to high tolerances at competitive rates. We also maintain a fully stocked material inventory for purchase, including steel, stainless steel, aluminum, brass, copper and plastics in a variety of forms and sizes. We also stock a large selection of metal working tools, o-rings, tube fittings and fasteners. Services include: Machining, Welding, Grinding, Turning, Milling, Wire and sinker EDM, Sheet metal fabrication, Vacuum leak testing, Heat treating, Gas brazing, Electro polishing, Fork Lift service for heavy equipment relocation.

Description: This shared resource is part of the Cooperative planning of the SDSU/UCSD Cancer Center Partnership grant. It is available to all SDSU and Cancer Center faculty and investigators conducting research related to cancer and under-represented ethnic groups.

Description: Cardiovascular Physiology Lab is a large animal (dog and pig) research facility with bi-plane x-ray imaging instrumentation, and cardiovascluar physiology measurement systems for blood pressure, vascular flow, ECG, etc. Core equipment includes GE bi-plane x-ray system and Gould amplifier system.

Description: CASS Research and Engineering Services and Test Facilities: The CASS thermal/vacuum facility is a vacuum chamber (4 ft diameter x 7 ft long) with porting for cold gas/LN2 interface to custom thermal shrouds with heaters for thermal control in the -150C to +50C range. Vacuum pressures of 10-6 Torr are achievable through the diffusion pump and cold trap. Automated control of the thermal system and cold trap nitrogen supply allows users to operate the system around the clock for cost effective thermal/vacuum environmental testing of space instrumentation. CASS engineers and programmers have decades of experience in instrument and system design for radiation detection and space applications. They have developed lung function instrumentation for the astronauts on the Space Shuttle, high resolution electron detectors for electron microscopy at the National Center for Microscopy and Imaging Research, as well as packaging phototubes for use in hostile environments deep undersea or in outer space.

Description: The Center currently has two state-of-the-art General Electric (GE) Discovery MR750 3T whole-body imaging systems for studies in humans and a Bruker 7T MRI system for animal studies. Access to these scanners is available on a recharge basis. The scanners are available for research scanning 24 hours a day / 7 days a week. Research scan time is scheduled through an online Webschedule system.

Description: The Center for the Future of Surgery, situated in the Medical Education and Telemedicine Building on the Medical School Campus, offers medical simulation and surgical training using the latest surgical technologies. We provide staffing and equipment for surgical testing, training, and research on a recharge basis. Studies can utilize large or small animals, or human cadavers across 22-stations in our standard OR suites, 15-station microsurgery suite, or a hybrid OR equipped with a c-arm for imaging.

Description: The Clinical Investigation and Biostatistics Core provides services and expertise to help investigators, especially those new to HIV research, to conduct clinical studies as well as to access longitudinal databases for epidemiologic and outcomes research. The Clinical Investigation and Biostatistics Core provides the expertise and resources necessary for basic, clinical, health services, and epidemiologic researchers to efficiently and safely conduct clinical research to increase our understanding and treatment of HIV disease. The Core also has access to longitudinal databases maintained by UC San Diego's Owen Clinic and the CFAR Network of Integrated Clinical Systems (CNICS).

The CB Core can assist you with design of data collection instruments; clinical trial design, randomization schemas, and training of clinical personnel; and statistical consultations, including sample size estimates, analysis plans, and statistical analyses.

Description: We provide consultation and services for the following: 1. Multiparameter cell phenotyping up to 11-color antibody combinations. 2. Analysis applications such as cell activation, proliferation, cell cycle/DNA content, apoptosis, surface marker expressions, intracellular expressions, quantification of internal, regulatory and structural proteins and quantification of reporter genes (GFP, DsRed, mCherry, etc). 3. High-speed cell sorting of live and biohazardous samples, single-cell deposition into 96-well plates. Core instruments include: MoFlo XDP sorter, FACSAria II sorter, FACSCanto analyzers.

Description: The Genomics and Sequencing Core is a full-service core facility providing services, reagents and expertise to perform assays ranging from small-scale to large-scale analysis of gene expression, microRNA quantitation, virus detection and quantitation, and SNP characterization, using TaqMan and Droplet Digital PCR technologies. We have over 15 years of experience in experimental design, consultation, nucleic acid extraction, cDNA synthesis, primer design, qPCR, and droplet digital PCR. Next generation sequencing for a variety of applications (bacterial genome, genome resequencing, miRNA, other) is available. Core equipment includes: Applied Biosystems 7900HT Fast Real-Time PCR System, Applied Biosystems 7500 Real-Time PCR System, two Bio-Rad QX100 Droplet Digital PCR Systems, Agilent 2100 Bioanalyzer & 2200 Tape Staion, Thermo Scientific NanoDrop ND-1000 and ND-8000 spectrophotometers, Qiagen QIAcube, Life Technologies Ion Torrent Personal Genome Machine and Illumina MiSeq sequencers.

Description: CFAR Translational Virology Core provides specimen processing, shipping, storage, nucleic acid preps, sequencing, analysis, and HIV clinical assays. Core equipment includes ABI sequencer, plate reader, 8 pcr thermocyclers.

Description: The Fabrication Facility, located in room 1610 of York Hall, is a full service development, renovation, demolition, and machine and wood shop. As a recharge facility, services may be extended to any of the academic departments at UCSD, as well as SIO.

Description: The Chemistry NMR facility features a 500MHz, two 400Mhz, and 300 MHz Varian spectrometers and is located in rooms 5140 and 6118 of Pacific Hall. The Biomolecular NMR facility features a Varian 800MHZ, a Bruker 600MHz and a Varian 500MHz and is located in B007 of the Natural Sciences Building. Reservations are made on a day-to-day basis. During normal business hours, sign-up time is limited to 45 minutes on the 300 and the two 400s. Experiments requiring more time should be performed during the evenings or weekends. For reservation limitations on the 500, please visit the 500 scheduling page. Please note that while all of the facility's spectrometers are capable of a standard battery of experiments, for non-standard applications, please identify which spectrometer fits your needs best before reserving time. If your project has time requirements that extend beyond the normal boundaries of what is available on the scheduler or you experience problems with the instruments, please contact us at n...@chem.ucsd.edu with your request. This will direct your query to everyone involved with maintaining the NMR facility. Core equipment includes Varian VNMRS 800 NMR with 3x Resonance Cold Probe (NSB), Varian VNMRS 500 NMR (NSB), Bruker Avance 600 NMR with 3x Resonance Cryoprobe (NSB), Varian VNMRS 500 NMR with Xsens Cold Probe (Pacific Hall), JEOL ECA 500 NMR (Pacific Hall), Varian Mercury Plus 400 NMR (Pacific Hall), Varian Mercury 400 NMR (Pacific Hall), and a Varian Mercury 300 NMR (Pacific Hall)

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