You have one solution you can make hot refference global and then you can use it in other function
var hot;
function Init(){
> hot = new Handsontable(container, {
> data: data,
> colWidths: [200, 200, 200],
> colHeaders: ['A', 'B', 'C'],
> contextMenu: false
> };
>
> }
>
> function Rebind(){
>
> //now you have instance directly availabe you can fire any method on it like hot.alter('remove_col', 0);
> }