Hi Ming-yi, that change will not result in a proper parsable GCT file, if that change worked then there is something else wrong with your file formatting that is causing it to erroneously be parsed without error.The proper formatting for the GCT format is described here: https://software.broadinstitute.org/cancer/software/gsea/wiki/index.php/Data_formats#GCT:_Gene_Cluster_Text_file_format_.28.2A.gct.29I'd also note that this mailing address is intended for gene set submissions, not for other support with GSEA. the GSEA-Help google group is the proper location for support inquiries.-AnthonyAnthony S. Castanza, PhDCurator, Molecular Signatures DatabaseMesirov Lab, Department of MedicineUniversity of California, San DiegoOn Thu, Jun 6, 2024 at 2:30 AM wrote:Hi,I am using GSEA for the first time. Following file format guidelines (Data Formats - GSEA-MSigDB Documentation), I encountered a "java.lang.NumberFormatException: For input string: "Description"" error. This error was solved by combining rows 1 and 2, that is,originally,#1.21000 130should have been#1.2 1000 130Thank you all for developing GSEA, a powerful and wonderful tool for analyzing expression data.Kind regards,Ming-yi