Hello,
I am reaching out in the context of my master’s thesis, which focuses on the proteomic analysis of head and neck cancers. The aim of my project is to investigate why HPV- cell lines produce more saBgal than HPV+ cell lines, taking into account the two HPV statuses specific to this cancer type.
I first analysed raw mass spectrometry data (DIA acquisition method) using DIA-Analyst, which allowed me to identify several relevant candidates. To better interpret these results, I then incorporated GSEA to highlight the associated signaling pathways.
However, I noticed an inconsistency: the fold change of a candidate in DIA-Analyst does not always match its enrichment score in GSEA. For example, focusing on trafficking in the HPV+ Rx vs HPV- Rx comparison (Rx = irradiation), some candidates with a high fold change and a relevant function (according to UniProt) do not show a high enrichment score in trafficking-related pathways in GSEA, contrary to my assumption.
I suspect there may be a methodological or conceptual aspect I am missing, and I would greatly value your insight on this matter.
Would you be willing to help me clarify this?
Kind regards,
Fatou