> What is the optimal number of markers in a map size of 778x640 with zoom
> level 13.
Performance degrades as markers are added. Some browsers and devices
do better than others. Acceptable performance is completely
subjective, if you will be having a lot of interaction with the map
your requirement may be more stringent than if it were a fairly static
display. So it's your decision. Most folk seem to feel that more
than a couple of hundred is unacceptable.
Visible clutter on the map increases as you place more markers, the
user is less able to take in the information at a glance. Small maps
and/or large or varied marker icons may exaggerate the problem. It's
subjective, so that's your decision.