The problem is that qplot draws the plot with the geom_point default geom, and then you are adding your geom_dotplot. So the points are being drawn twice. I would suggest using ggplot() rather than qplot(), so the only time they are drawn is when you explicitly add the dotplot layer eg
ggplot(data=tt, aes(x=pathway,y=fc)) + geom_dotplot(binaxis="y", aes(fill=factor(de)), colour=NA, binwidth=0.01,binstataxis="y")
cheers
PE
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