Bowtie indexer error

[Fernando Alemán]

Hi there,
Before running the Bowtie Aligner I need a Bowtie Index 2 as a template. There is none publicly available for tomato so I am creating a custom template (a Bowtie Index) with the Bowtie Indexer. (I hope that's what I have to do to work with tomato RNA-seq samples).

So I input a fasta file as requested and leave the rest as default, including the random seed number (=123456). 

I click run and got this error message:

"The random seed must be an integer"

I don't know what went wrong nor how to solve it. 

Thanks,

Fernando.

[Barbara Hill]

Hello Fernando, 

Do you have a job id? This would make it easier for us to investigate.

Best

-Barbara

[Fernando Alemán]

Hi Barbara,
Thanks for your reply. I tried to find the job ID but there are too many (failed) jobs and couldn't find it. 
So I went again to the Bowtie Indexer and repeat the process with a fasta file of all cDNA from tomato (from Emsembl) and left the rest on default (I hope that's what I had to do).

The new job ID is 1602025 and I will keep you posted (it is pending and taking quite a long time).

Thanks again and happy holidays!

Fernando. 

[Fernando Alemán]

I think the new job 1602025 worked now!

Thanks!

Fernando.