ERROR:5 question

[tarawi...@gmail.com]

Hello,

I have a question about a job I ran which resulted in an ERROR:5 (JobID:86136), which says that no significant SNPs were identified. However, my input file consists of 54 SNPs that have already been identified as significant by another article. So this cannot be the problem. 

Below is a copy of part of my input file:

rsID Allele1 Allele2 Effect StdErr Pvalue CHROM POS N

rs4246511 c t -0.22 0.02 5.1E-21 1 39152972 69116

rs12142240 t c -0.13 0.02 6.6E-09 1 46519888 69356

rs1411478 a g -0.13 0.02 1.4E-10 1 179228905 68680

rs2236918 c g -0.15 0.02 8.3E-14 1 240084449 69332

rs704795 a g -0.16 0.02 2.1E-15 2 27569998 69341

rs1800932 a g -0.17 0.03 3.2E-11 2 47871585 69309

rs930036 a g -0.19 0.02 3.1E-19 2 171649264 69357

rs16858210 g a -0.14 0.02 3.1E-09 3 185106704 69193

rs4693089 a g -0.2 0.02 9.2E-23 4 84592646 69060

rs6856693 a g -0.16 0.02 9.8E-15 4 185985800 67635

A colleague of mine suggested that it might be a problem with the Pvalue in the input file. 

I now changed it to the same format, but the capital E replaced by a small one. This job is running now (JobID:86414). I don't know if this was the problem, and if this then is the solution. Do you know this? 

(I will post an update once it ran)

Thanks in advance.

rsID Allele1 Allele2 Effect StdErr Pvalue CHROM POS N

rs4246511 c t -0.22 0.02 5.094e-21 1 39152972 69116

rs12142240 t c -0.13 0.02 6.618e-09 1 46519888 69356

rs1411478 a g -0.13 0.02 1.355e-10 1 179228905 68680

rs2236918 c g -0.15 0.02 8.309e-14 1 240084449 69332

rs704795 a g -0.16 0.02 2.077e-15 2 27569998 69341

rs1800932 a g -0.17 0.03 3.180e-11 2 47871585 69309

rs930036 a g -0.19 0.02 3.073e-19 2 171649264 69357

rs16858210 g a -0.14 0.02 3.069e-09 3 185106704 69193

rs4693089 a g -0.2 0.02 9.162e-23 4 84592646 69060

rs6856693 a g -0.16 0.02 9.786e-15 4 185985800 67635

[tarawi...@gmail.com]

The job again gives an ERROR:5 notification... I do not know what I am doing wrong unfortunately.

[Kyoko Watanabe]

Hi,

I am sorry for my slow response.

I had a look at your jobs.

I do not have access to your input file but I can see some of the outputs.

FUMA can only annotate SNPs present in the selected reference panel, which means chr, position and alleles have to match with the reference panel.

Currently FUMA only supports GRCh37.

SNPs position in your file does not look like either GRCh37 nor GRCh38.

Could you check the build and map to GRCh37 then try again?

Best,

Kyoko