Three nucleic acid probes, two for autotrophic ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria and one for alpha subdivision nitrite-oxidizing bacteria, were developed and used to study nitrifying bacterial phylotypes associated with various freshwater and seawater aquarium biofilters. Nitrosomonas europaea and related species were detected in all nitrifying seawater systems and accounted for as much as 20% of the total eubacterial rRNA. In contrast, nitrifying bacteria belonging to the beta-proteobacterial subdivision were detected in only two samples from freshwater aquaria showing vigorous nitrification rates. rRNA originating from nitrite-oxidizing alpha subdivision proteobacteria was not detected in samples from either aquarium environment. The data obtained indicate that chemolithotrophic ammonia oxidation in the freshwater aquaria was not due to beta-proteobacterial phylotypes related to members of the genus Nitrosomonas and their close relatives, the organisms usually implicated in freshwater nitrification. It is likely that nitrification in natural environments is even more complex than nitrification in these simple systems and is less well characterized with regard to the microorganisms responsible.
Eligible organizations may include, but are not necessarily limited to: zoos, botanical gardens, arboretums, aquaria, bird sanctuaries, natural habitat preserves, natural science museums, and nature and environmental centers.
We are the oldest living aquarium web site, having been around sinceJanuary 1994. If this is your first time, you may wish to look at thedetailed subject index. It willhelp you get an idea of where to find things here. Don't be fooled bythe simple front page... there are over 38 megabytes of information stored here!What's New?Nothing! I don't really update the site. But for old old updates, see Change Summary for a detailed day-by-daydescription of new and changed pages. What's in a Name? If you're interested, here's some backgroundon this site, its history, name, etc. I also maintain a couplerelated sites you may also enjoy: the Greater Seattle Aquarium Society pages,which features an archive of our locally-grown articles and photos; the Aquatic GardenersAssociation, dedicated to the keeping of aquatic plants in theaquarium and pond; and also the FAQ (see below).Additional Resources...The Aquaria FAQ(Originally written for the newsgroups rec.aquaria.*)is an excellent starting point for learning about the aquarium hobby. Lookfor hooks into specific parts of the FAQs where you see the FAQ logo.Some smaller and detailedsites web sites are found in the subject index underOrganizations, and where appropriate toa topic on other pages (look for the logo). The fine print:Articles, images and photos are copyrighted by theirrespective authors. Many texts are public-domain Usenet postings, but in general, please ask (e-mail) the authors beforeredistributing.All e-mail addresses herehave the @ (at) sign replaced by a / (slash) as or the word "-at-", a spam preventative. You will have to manually type theaddress in to e-mail the person. I hate spam, and hate the idea of it being sent to someone because theyonce wrote an article here.
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