Please help with projection

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Natalia Campos

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May 20, 2025, 10:22:20 AM5/20/25

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Dear flashpca users:

My name is natalia. Many thanks beforehand. i have been trying to use flashpca to project existing PCs onto new samples. However, there is a mistake somewhere because i cannot get the projections.txt file.

i have previously run flashpca to estimate PC and save meansd.txt and loadings.txt

Then i run

flashpca --bfile file --project --inmeansd meansd.txt --outproj projections.txt --inload loadings.txt

However, i get this output and the command is interrumpted and i do not get the output files:

[Tue May 20 16:03:00 2025] arguments: flashpca flashpca --bfile DataChrAllRelatedForPC -d 20 --div p --project --inmeansd meansd.txt --inload loadings.txt -v --outproj projections.txt

[Tue May 20 16:03:00 2025] Start flashpca (version 2.0)

[Tue May 20 16:03:00 2025] seed: 1

[Tue May 20 16:03:00 2025] Detected bim file DataChrAllRelatedForPC.bim, 26695 SNPs

[Tue May 20 16:03:00 2025] Analyzing BED file 'DataChrAllRelatedForPC.bed', found 545672498 bytes, 26695 SNPs

[Tue May 20 16:03:00 2025] Detected BED file: DataChrAllRelatedForPC.bed with 545672498 bytes, 81764 samples, 26695 SNPs.

[Tue May 20 16:03:00 2025] mem: 2147483648 mem_req_bytes: 52198832 mem_remain_bytes: 2095284816

[Tue May 20 16:03:00 2025] Reserving 52198832 bytes, leaving 2095284816 bytes for SNP blocks

[Tue May 20 16:03:00 2025] blocksize: 3203 (2095120736 bytes per block)

[Tue May 20 16:03:00 2025] Reading mean/stdev file meansd.txt

MAny thanks!! your help will be highly appreciated.

MAY ALMIGHTY GOD bless you all.

natalia