Fit-Hi-C for Region Capture-Hi-C

[Guillaume HEGER]

Hello,

I am seeking to assign significance to pairs interactions of several Region Capture-Hi-C datasets. Is Fit-Hi-C adapted to this type of data? Are there any extra precaution that should be taken? In particular, is the use of a biasfile still recommended in this case or is it specific to genome-wide Hi-C?

Thanks!

[Ferhat Ay]

Hi, yes we have previously applied it to region capture data (Sima et al. Cell 2018). I do not think we needed to change anything to make it work. You will just have to make sure you are only using the data from the captured region. If you use all data then almost everything in the capture regions will look significantly, as you can guess.

 

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