Free Download Miracle Box Crack 2.58

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Aug 3, 2024, 10:49:54 AM8/3/24
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Industrial activities have a detrimental impact on the environment and health when high concentrations of pollutants are released. Phytoremediation is a natural method of utilizing plants to remove contaminants from the soil. The goal of this study was to investigate the ability of Cannabis sativa L. to sustainably grow and remediate abandoned coal mine land soils in Pennsylvania. In this study, six different varieties of industrial hemp (Fedora 17, Felina 32, Ferimon, Futura 75, Santhica 27, and USO 31) were grown on two different contaminated soil types and two commercial soils (Miracle-Gro Potting Mix and PRO-MIX HP Mycorrhizae High Porosity Grower Mix). Plants growing in all soil types were exposed to two environmental conditions (outside and in the greenhouse). Seed germination response and plant height indicated no significant differences among all hemp varieties grown in different soils, however on an average, the height of the plants grown in the greenhouse exceeded that of the plants grown outdoors. In addition, heavy metal analysis of Arsenic, Lead, Nickel, Mercury, and Cadmium was performed. The concentration of Nickel was 2.54 times greater in the leaves of hemp grown in mine land soil outdoors when compared to greenhouse conditions. No differences were found between expression of heavy metal transporter genes. Secondary metabolite analysis of floral buds from hemp grown in mine land soil displayed a significant increase in the total Cannabidiol content (2.16%, 2.58%) when compared to Miracle-Gro control soil (1.08%, 1.6%) for outdoors and in the greenhouse, respectively. Molecular analysis using qRT-PCR indicated an 18-fold increase in the expression of the cannabidiolic acid synthase gene in plants grown on mine land soil. The data indicates a high tolerance to heavy metals as indicated from the physiological and metabolites analysis.

Copyright: 2019 Husain et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This work received financial support by the National Science Foundation, grant number 1560049 ( ) to RH. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Depletion of the ozone layer and global warming are two of the biggest issues at hand due to the release of toxic pollutants into the environment [1]. Inorganic pollutants include heavy metals such as Fe, Mn, Zn, Cu, Mg, Mo, and Ni, which are necessary for plant growth but are detrimental to the environment at high concentrations in the soil. Leaching of these metals into surrounding areas through rainwater runoff poses a dangerous environmental and health risk [2]. Metals with an unknown biological purpose such as Cd, Cr, Pb, Co, Ag, Se, and Hg can also become accumulated and in high amounts be toxic [3]. Phytoremediation is a natural, cost-effective process in which plants are used to remove unsafe compounds from the soil [4], [5]. Many plants have been studied and deemed effective at removing toxins from the soil. However, only a small number of those plants have numerous beneficial applications outside of phytoremediation. Methods of extraction and disposal of these plants have not been well defined [2], [6].

The purpose of this study was to explore the potential of industrial hemp as an eco-friendly way to remediate abandoned mine land soils in Pennsylvania. To do that, physiological parameters such as seed germination, plant height, and days-to-flowering were examined. In addition, uptake of heavy metals such as Arsenic, Lead, Nickel, Mercury, and Cadmium, changes in soil pH, and total cannabinoid content were examined in six different varieties of industrial hemp (Fedora 17, Felina 32, Ferimon, Futura 75, Santhica 27, and USO 31) grown on two contaminated soil types and two commercial soil types both outdoors and in the greenhouse. Additionally, this study presents a detailed review on the possible mechanisms of metal uptake.

Four different soil types, mine land 1 (New Port, PA, USA), mine land 2 (New Port, PA, USA), Miracle-Gro Potting Mix (Marysville, OH, USA), and PRO-MIX HP Mycorrhizae High Porosity Grower Mix (Rivire-du-Loop, Qubec, Canada) were used in this study. For every soil type except Miracle-Gro Potting Mix, three individual pots for each of the six hemp varieties were used making it a total of 18 pots per soil type. For the Miracle-Gro Potting Mix, two individual pots for each of the six hemp varieties were used making it a total of 12 pots. This entire set up of 66 pots representing all four soil types was duplicated for both outside and inside the greenhouse making it a total of 132 pots for the entire study.

Seed germination was recorded after one week of planting. Plant height was recorded weekly over the course of nine weeks and days to flowering was monitored as indicated by the formation of the first flower buds.

Soil samples (Miracle-Gro and mine land 1 soil from outdoors and in the greenhouse) and the leaves closest to the soil from each individual plant within each treatment were collected for heavy metal analysis in triplicates. The Department of Environmental Protection (DEP) Chapter 271 General Soil Permit test was performed on the initial soil samples from these two soil types by the Agricultural Analytical Services Laboratory (University Park, PA). The test performed screened for Arsenic, Cadmium, Copper, Lead, Mercury, Molybdenum, Nickel, Selenium, Zinc, and polychlorinated biphenyls to satisfy the requirements of DEP Chapter 271, General Permit for Land Application of Sewage Sludge. The test also measured the soil pH. Three biological replicates per treatment were combined and digested [12] using the Environmental Express AutoBlock Plus for acid digestion of soil. Samples were analyzed using the Varian Inc. 720/730-ES Inductively Coupled Plasma-Optical Emission Spectrometer (ICP-OES) with axial plasma following EPA (Environmental Protection Agency) method 6010 for Arsenic, Lead, Nickel, Mercury, and Cadmium.

Leaf samples were harvested in three biological replicates per treatment, flash frozen in liquid nitrogen, stored in the -80C freezer [13], and prepared for heavy metal analysis using (EPA method 3051) Microwave Assisted Acid Digestion at the ALS Laboratory (Middletown, PA). Samples were then analyzed for Arsenic, Lead, Nickel, Mercury, and Cadmium using Manual Cold-Vapor Technique and Inductively Coupled Plasma-Mass Spectrometry (EPA methods 6020A and 7471B).

Floral buds from 70-day-old plants from variety Fedora 17 were analyzed for cannabinoid potency by measuring total cannabidiol (CBD) and Δ9- tetrahydrocannabinol (THC) at the Analytical 360 Laboratory (Seattle, Washington). A total quantity of 500 mg of floral bud tissue from the Fedora 17 variety was harvested from indoor and outdoor plants grown on mine land 1 or Miracle-Gro soils. Methanol was used to extract the secondary metabolite compounds. The plant material was then analyzed using the Hewlett-Packard 1050 High Performance Liquid Chromatography (HPLC) equipment based on the De Backer method [14].

The events associated with seed germination reflects the uptake of water from the soil leading to the emergence of the radicle and visible extension of the shoot [17]. Seed germination has been shown to be influenced by acidic pH due to rapid uptake of nutrients by the roots during the phase of seedling growth [18]. Seed germination data was collected one-week post planting as shown in Table 4.

The presence of heavy metals in the soil impacts physiological and biochemical processes, thus influencing the overall uptake of nutrients, altering plant growth significantly [20], [21]. Plant height is a critical factor for fiber yield, allowing more surface area for metals to accumulate for phytoremediation. Plant height data was collected weekly for a total period of nine weeks. Average plant height at nine weeks is shown in Table 5.

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