Synchronizing FED3 with Doric Fiber Photometry

[Joao Alfredo]

Hi everyone,

I am planning to synchronize a FED3 device with a Doric Fiber Photometry system using the FED3 TTL BNC output.

I was wondering if anyone has experience using this combination and would be willing to share their workflow.

I have a few questions:

1. How are the TTL pulses generated by the FED3 displayed in the Doric acquisition software? Are they shown on the same timeline as the fluorescence signals?
2. When exporting the photometry data, are the behavioral TTL events included in the same data file as the fluorescence signals, or do they need to be exported separately and merged afterward?
3. After the recording, how do you synchronize the behavioral events (pellet retrieval, pokes, etc.) with the fluorescence data? What workflow do you typically use?
4. Does anyone have a Python script (or other code) for aligning FED3 behavioral events with Doric fiber photometry recordings that they would be willing to share?
5. If there are any tutorials, GitHub repositories, or example analysis pipelines for FED3 + Doric synchronization, I would greatly appreciate any recommendations.

We are planning to start this experiment soon, so I am trying to establish a reliable analysis workflow before collecting data.

Thank you very much for your help!

Best regards,

João