There is no single temperature that will determine layer height. It is
very dependent on your ambient and boundary conditions. Wall
conductivity, specific heat, density, and thickness all play into the
equation for heat removal (driving the upper gas layer temperature)
and the ambient air temp is generally used for the lower gas layer
temperature (in two zone models). Ventilation parameters will also
greatly affect the layer height. If you use a slice file along with
the layer height data, you may be able to pull a temperature that will
more or less give you a temperature to use for that specific model,
but I wouldn't put too much stock into that number.
If you're uncertain as to what the layer height is and what affects
it, I would suggest playing around with the Fire Dynamics Tools (FDTs)
to get a bit more familiar with it. They're just spreadsheet tools
that assume a steady state fire and use algebraic correlations to give
an estimate of properties.
http://www.nrc.gov/reading-rm/doc-collections/nuregs/staff/sr1805/
You can also play around with CFAST and it will use smokeview to
illustrate the layer height.
If you have a more specific question about the layer height or hot gas
layer, I'd be happy to try to answer it, but from your original post,
it's difficult to know exactly what you're asking. I don't know what
you're using for "height of the level of smoke in the room" or what
the difference is that you are seeing. Hopefully I've helped a bit,
but it's hard to answer a general question when there is so much that
plays into the phenomena.