#t2ac: FATAL ERR: 30568 duplicate ids in transcripts.aa

[Karen Daiana Schoninger]

I want to run evigene tr2aacds4.pl with three assemblies from TransAbyss.

I run the command:

tr2aacds4.pl -log -cdna transcripts.fa -NCPU 24 -MAXMEM 16000 -MINAA=35 -lo
gfile _evigene.log

And the result is:

#t2ac: FATAL ERR: 30568 duplicate ids in transcripts.aa

Could you guide me? 

Thank you,

Karen :)

[Don Gilbert]

You want to use trformat.pl to ensure unique ids for your input transcript set:

evigene/scripts/rnaseq/trformat.pl

  Use BEFORE tr2aacds to regularize IDs in fasta of Velvet,Soap,Trinity, ensure unique IDs, add prefixes for parameter sets.