question regarding the upload of a FASTA alignment file

[afonso...@gmail.com]

Dear All,

I have a allignment file that I want to use to calculate the EVcouplings. This file includes some expecially picked sequences that were not picked by the EVcouplings website so I want to try it in the server.

I uploaded the file in the "upload alignment" and insert the sequence of the target protein in the "Enter Your Protein".
However when I run it i get the error:

"progress stopped in : making/finding family alignment"

I formatted the FASTA file as indicated, what could it be the problem ?

Best

Afonso

[afonso...@gmail.com]

I'm including the 2 first seq in the alignment just to illustrate it:
"

>gi1
-----------------------M--NMLALTIILPLIGFVLL---------AFSRGRW-
---SENVS-AIVGVGSVGLAALVTAFIGVD------FFAN----GEQA------------
-YSQPLWTWM-------SVG--------D------FNIGFNLVLDGLSLTMLSVVTGVGF
LIHMYASWYMRGEE-----------G-YS-----RFFAYTNLFIASMVVLVLADNLLLMY
LGWEGVGLCSYLLIGFYYTDPK-------NGAAAMKAFVVTRVGDVFLAFALFILYNELG
------TLNFREMVELAPA-HFADG-------NN------MLMWATLMLLGGAVGKSAQL
PLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHL-VGIVGAVTLL
LAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQ--AWDAAIF---------HLMTHAFF
KALLFLASGSVILACHH----------------E-QNIFKMGGLRKSIPLVYLCFLVGGA
ALSALPLVTAGFFSKDEILAG----AMANGHI-----------NLMVAGLVGAFMTSLYT
FRMIFIVFHG------------KEQ----------IHAHAV----KGVTHSLPLIVLLIL
STFVGALIVPP----------------LQGVL-------PQT----TEL-----AHG---
SM-LT--LEITSGVVAVVGILLA-AW-LW-LGKR-TLVTSI-ANSAPGRL---LGTWWYN
AWGFDWLYDKVFVKPFLG----------------------------IAWLLKR-------
--DPLNS-MMNI-PAVLSRFAGKGLLLS---------------------------ENGYL
RWYVASMSIGAVVVLALL-MVLR-------------------------------------
------------------------------------------------------------
----------------
>gi2
-----------------------M--NMLALTIILPLIGFVLL---------AFSRGRW-
---SENLS-ATVGVGSIGLAALVTAFVGVD------FFAN----GKQA------------
-FSQPLWTWM-------SVG--------D------FNIGFNLVLDGLSLTMLSVVTGVGF
LIHMFASWYMRGEE-----------G-YS-----RFFAYTNLFIASMVILVLSDNLLLMY
LGWEGVGLCSYLLIGFYYTDPK-------NGAAAMKAFVVTRVGDVFLAFALFILYNELG
------TLNFREMVELAPA-HFADG-------NN------MLMWATLMLLGGAVGKSAQL
PLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHL-VGIVGAITLV
LAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQ--AWDAAIF---------HLMTHAFF
KALLFLASGSVILACHH----------------E-QNIFKMGGLRKSIPLVYLCFLVGGA
ALSALPLITAGFFSKDEILAG----AMANGHI-----------NLMVAGLVGAFMTSLYT
FRMIFIVFHG------------KEQ----------IHAHAG----KGITHHLPLIVLMVL
STFVGALIVPP----------------LQGVL-------PQT----TEL-----EHS---
RV-MT--LEIASGVVAIVGILIA-AW-LW-LGKR-TLVTSV-ANSAPGRL---LGTWWYN
AWGFDWLYDKVFVKPFLG----------------------------VAWLLKR-------
--DPLNA-LMNV-PAILSRFAGKGLLLS---------------------------ENGYL
RWYVASMSIGAVVVLALL-MVLR-------------------------------------
------------------------------------------------------------
----------------

"

[armi...@gmail.com]

Hello,

Has the been solved? I am experiencing the same problem.

Thanks,
Armin