A promiscuous enzymatic synthesis technique for DNA data storage
18 views
Skip to first unread message
Bryan Bishop
Nov 20, 2022, 9:37:30 AM11/20/22
to enzymatic...@googlegroups.com, Bryan Bishop
Hi all,
My coinventors and I did some work on "promiscuous templates" and "promiscuous polymerase" as a way of encoding data into DNA molecules.
"""
In some embodiments, a polymerase can be responsive to environmental effectors. Such responsiveness can be a native property of the polymerase or the polymerase can be engineered to have such a response. By way of example, but not limitation, the “error” rate of certain polymerases can be modified by environmental effectors such as ionic concentration, electrical stimuli, chemical stimuli, optical stimuli, non-optical radiation, temperature modulation and pH modulation. The speed or nucleotide preference of such polymerase can also, in certain aspects, be affected by the same environmental effectors. In some embodiments, where the “error” rate of a polymerase is increased by an environmental effector, a promiscuous nucleotide(s) can be added to a reaction mixture such that the polymerase has an increased probability of incorporating the promiscuous nucleotide as opposed to the canonical nucleotide when replicating a template nucleic acid molecule. This increase can, as defined herein, be used to encode information in the encoded nucleic acid molecule.
"""
Read more here:
My coinventors and I did some work on "promiscuous templates" and "promiscuous polymerase" as a way of encoding data into DNA molecules.
"""
In some embodiments, a polymerase can be responsive to environmental effectors. Such responsiveness can be a native property of the polymerase or the polymerase can be engineered to have such a response. By way of example, but not limitation, the “error” rate of certain polymerases can be modified by environmental effectors such as ionic concentration, electrical stimuli, chemical stimuli, optical stimuli, non-optical radiation, temperature modulation and pH modulation. The speed or nucleotide preference of such polymerase can also, in certain aspects, be affected by the same environmental effectors. In some embodiments, where the “error” rate of a polymerase is increased by an environmental effector, a promiscuous nucleotide(s) can be added to a reaction mixture such that the polymerase has an increased probability of incorporating the promiscuous nucleotide as opposed to the canonical nucleotide when replicating a template nucleic acid molecule. This increase can, as defined herein, be used to encode information in the encoded nucleic acid molecule.
"""
Read more here:
- Bryan
https://twitter.com/kanzure
https://twitter.com/kanzure
Reply all
Reply to author
Forward
0 new messages