Re: New to helical reconstruction and SPRING

[Carsten Sachse]

Dear Kaiming,

On 31 Jan 2014, at 04:18, kmzhang <kaiming...@gmail.com> wrote:

Hi, Carsten, 

     I have tried to use the software, I downloaded the tutorial  from the Spring HOME. 

But from the .pdf, I am not very clear how to set the parameters with my own project. 

Will you have a workshop recently? Maybe just because I have no experience with 

the helical reconstruction. 

If you are new to helical reconstruction I would set up a TMV run using the tutorial to learn how to use SPRING. The default (TMV) parameters are most of the time very good starting estimates for your project. There are 'beginner', 'intermediate' and 'expert' parameters. 'Beginner' parameters are essential to adapt for your project, e.g. input files. Default 'intermediate' and 'expert' should will work for most projects.

Best wishes,

Carsten 

   Regards

   Kaiming

KaiMing Zhang, Ph.D candidate

Macrobiomolecular Complex Labrotaroy

Department of Biophysics 

Health Science Centre

Peking University

#38 Xueyuan Road

Beijing 100191 

China

On Jan 30, 2014, at 1:14 PM, Carsten Sachse <carsten...@embl.de> wrote:

Dear Kaiming,

Glad to hear that it worked.

Fibrils will work too. Maybe not to the highest resolution. 

Best wishes,

Carsten

On 29 Jan 2014, at 18:26, kmzhang <kaiming...@gmail.com> wrote:

Hi, Carsten,

     I am happy to tell you I finally install Spring successfully.  I re-installed everything and removed macport and fink, then everything is OK. And I found you gave the more details about the installation  in the Spring home, which is more helpful. 

     Now I am trying to use it,  do you think the fibrils (not straight) can be reconstructed using this software?

     Regards

    Kaiming

On Jan 27, 2014, at 3:11 PM, kmzhang <kaiming...@gmail.com> wrote:

Hi, Carsten,