segclassexam

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cyanpku

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Feb 19, 2016, 8:08:36 AM2/19/16
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Hi Carsten,
Thank you very much for explaining how the segment refine3D works!
Another issue of spring that confused me:
The "power spectrum resolution cutoff" option seems to rescale the pixel size for measuring the helix width and helical normal. I keep the pixel size in A the same as 3.24A. If I set the cutoff at 0.05, the hlx width reads 46A, and if I change the cutoff to 0.1, the hlx width goes up to 105A. Both of which are not the true width sine it should be something around 220 A. 
When I use resolution cutoff higher than 0.1 (0.11), there's attribute error:

AttributeError: 'SegClassExam' object has no attribute 'pixelsize'


Would you please help me sort it out so that I can have a accurate reading of the hlx width?


Thank you very much!


Jing


cyanpku

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Feb 19, 2016, 8:26:00 AM2/19/16
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Oops, just found out it's a bug fixed in the newer version of spring. I'll go update!
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