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by by {"isAjaxInProgress_B01F4WT8IS":"0","isAjaxComplete_B01F4WT8IS":"0"} Xinkun Wang (Author) › Visit Amazon's Xinkun Wang Page Find all the books, read about the author, and more. See search results for this author Are you an author? Learn about Author Central Xinkun Wang (Author).
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Description
A Practical Guide to the Highly Dynamic Area of Massively Parallel Sequencing The development of genome and transcriptome sequencing technologies has led to a paradigm shift in life science research and disease diagnosis and prevention. Scientists are now able to see how human diseases and phenotypic changes are connected to DNA mutation, polymorphism, genome structure, and epigenomic abnormality. Next-Generation Sequencing Data Analysis shows how next-generation sequencing (NGS) technologies are applied to transform nearly all aspects of biological research. The book walks readers through the multiple stages of NGS data generation and analysis in an easy-to-follow fashion. It covers every step in each stage, from the planning stage of experimental design, sample processing, sequencing strategy formulation, the early stage of base calling, reads quality check and data preprocessing to the intermediate stage of mapping reads to a reference genome and normalization to more advanced stages specific to each application. All major applications of NGS are covered, including: RNA-seq: mRNA-seq and small RNA-seq Genotyping and variant discovery through genome re-sequencing De novo genome assembly ChIP-seq to study DNA–protein interaction Methylated DNA sequencing on epigenetic regulation Metagenome analysis through community genome shotgun sequencing Before detailing the analytic steps for each of these applications, the book presents the ins and outs of the most widely used NGS platforms, with side-by-side comparisons of key technical aspects. This helps practitioners decide which platform to use for a particular project. The book also offers a perspective on the development of DNA sequencing technologies, from Sanger to future-generation sequencing technologies. The book discusses concepts and principles that underlie each analytic step, along with software tools for implementation. It highlights key features of the tools while omitting tedious details to provide an easy-to-follow guide for practitioners in life sciences, bioinformatics, and biostatistics. In addition, references to detailed descriptions of the tools are given for further reading if needed. The accompanying website for the book provides step-by-step, real-world examples of how to apply the tools covered in the text to research projects. All the tools are freely available to academic users.
Let's be real: 2020 has been a nightmare. Between the political unrest and novel coronavirus (COVID-19) pandemic, it's difficult to look back on the year and find something, anything, that was a potential bright spot in an otherwise turbulent trip around the sun. Luckily, there were a few bright spots: namely, some of the excellent works of military history and analysis, fiction and non-fiction, novels and graphic novels that we've absorbed over the last year.
Here's a brief list of some of the best books we read here at Task & Purpose in the last year. Have a recommendation of your own? Send an email to ja...@taskandpurpose.Com and we'll include it in a future story.
Missionaries by Phil Klay
I loved Phil Klay’s first book, Redeployment (which won the National Book Award), so Missionaries was high on my list of must-reads when it came out in October. It took Klay six years to research and write the book, which follows four characters in Colombia who come together in the shadow of our post-9/11 wars. As Klay’s prophetic novel shows, the machinery of technology, drones, and targeted killings that was built on the Middle East battlefield will continue to grow in far-flung lands that rarely garner headlines. [Buy]
- Paul Szoldra, editor-in-chief
Battle Born: Lapis Lazuli by Max Uriarte
Written by 'Terminal Lance' creator Maximilian Uriarte, this full-length graphic novel follows a Marine infantry squad on a bloody odyssey through the mountain reaches of northern Afghanistan. The full-color comic is basically 'Conan the Barbarian' in MARPAT. [Buy]