Tomogram preprocessing produces a file with 16 slices

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Jaime Llodra

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Aug 19, 2021, 5:28:44 AM8/19/21

to EMAN2

Dear Group Members,

After the tomogram preprocessing the resulting file has only 16 slices. Is this a default setting? Do you know how to get a file with original number of slices?

Thanks in advance for your help and support.

Jaime Llodra

Ludtke, Steven J.

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Aug 19, 2021, 8:33:51 AM8/19/21

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Hi Jaime,

you need to be a lot more precise in your questions. You aren't providing enough information for anyone to help without going back and forth several times.

Tomogram preprocessing should produce files the same size as the inputs. What are the dimensions of the input tomogram? What are the dimensions of the output tomogram? What is the exact command you executed (from the Command tab)?

Anyway, we're happy to help you debug issues, but you need to give us as complete information as possible. If something isn't working, there are 3 basic possibilities:

1) something is wrong with the input file(s)

2) something is wrong with the options given to the command

3) an actual bug in the system

The vast majority of problems people have during one of the tutorials are due to users accidentally mis-reading the tutorial. Occasionally there are actual bugs in the software that someone finds, but before we release the software we try to make sure we have tested everything used in the tutorials to avoid exactly this sort of problem.

--------------------------------------------------------------------------------------

Steven Ludtke, Ph.D. <slu...@bcm.edu>                      Baylor College of Medicine 
Charles C. Bell Jr., Professor of Structural Biology

Dept. of Biochemistry and Molecular Biology                      (www.bcm.edu/biochem)
Academic Director, CryoEM Core                                        (cryoem.bcm.edu)

Co-Director CIBR Center                                    (www.bcm.edu/research/cibr)

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Jaime Llodra

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Aug 19, 2021, 12:08:15 PM8/19/21

to EMAN2

Dear Members of this group,

Here is a more detailed description of what I am doing and the datasets that result from the use of the Preprocess tomograms option of EMAN2

- I import the tomogram which in fact is a dataset from a SBF-SEM machine which generates a stack of slices (.tif extension). The file has 512 X 512 X 926 dimensions (XYZ) and a size of 248 MB

- After importing the tomogram I get one file with the hdf extension in the tomograms folder with a size of 16.8 MB

- The preprocessing of the previous file generates a _preproc.hdf file with a size of 16.8 MB and 512 X 512 X 16 dimensions

- The _preproc.hdf file can be used for the Box training references step

Thanks for any help and support

Jaime Llodra

Ludtke, Steven J.

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Aug 20, 2021, 6:53:11 PM8/20/21

to em...@googlegroups.com

Ok, a few points:

- there are some significant differences between a stack of slices and a tomogram (generated from transmission images of tilts). The contrast will be very different, and the Z continuity (assuming the slices are x/y) in z will be very different than you get in tomograms. You should still be able to get segmentation to work, but it isn't completely clear what issues you might encounter.

- Just so you are aware, tomograms are typically substantially shorter in the Z dimension than x/y. While this isn't necessarily a problem, it is possible that some bugs may arise if specific programs make some assumptions along those lines.

On Aug 19, 2021, at 11:08 AM, Jaime Llodra <jaime...@gmail.com> wrote:

Dear Members of this group,

Here is a more detailed description of what I am doing and the datasets that result from the use of the Preprocess tomograms option of EMAN2

- I import the tomogram which in fact is a dataset from a SBF-SEM machine which generates a stack of slices (.tif extension). The file has 512 X 512 X 926 dimensions (XYZ) and a size of 248 MB

- After importing the tomogram I get one file with the hdf extension in the tomograms folder with a size of 16.8 MB

The smaller file size doesn't necessarily indicate a problem. We use compression when data is imported into the system, so it may become significantly smaller, though that is quite a substantial reduction, so there may well be a problem during import. Did you look at the dimensions of the image after importing but before preprocessing (e2display.py browser will show image dimensions)? If it is the correct size then we can try to figure out what went wrong in the next step. If it is the wrong size then we need to figure out what went wrong with that. The standard TIFF file format does not support 3-D data, but it does support sets of 2-D images, so that is the typical representation that might be used for this sort of data. However, there are a lot of odd things companies may do with tiff files which could cause difficulties. If the imported volume dimensions are wrong you might try running tiffinfo (not part of EMAN2) on one of the TIFF volumes and post the output here.

- The preprocessing of the previous file generates a _preproc.hdf file with a size of 16.8 MB and 512 X 512 X 16 dimensions

If the imported file had the correct dimensions but they are wrong after preprocessing, again, we need to see the exact command you ran to do the import. You can see the commands via the "Command" tab in e2projectmanager or in the .eman2log.txt file

- The _preproc.hdf file can be used for the Box training references step

Not sure what this means. Are the 16 slices you have all good? Are you just missing the other 910 slices?

To view this discussion on the web visit https://groups.google.com/d/msgid/eman2/6e33a854-6f99-443b-a0b4-aca60b30edben%40googlegroups.com.

Jaime Llodra

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Aug 23, 2021, 5:33:08 AM8/23/21

to EMAN2

Dear Members,

I am aware that my dataset is not a tomogram. It is a stack of Z slices
collected by an SEM. Having said that I have to tell you that years ago I
used EMAN2 to process a similar dataset for object classification. This
file had a .mrc extension and was processed fine. The current file I am
having problems with has a .tif extension and maybe that is problem. I will
use the original files which are in the .dm4 format and see how EMAN2
behaves.

Here is the output of tiffinfo

Last login: Mon Aug 23 09:57:31 on ttys001

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(base) TUAP227:~ jl2235$ cd Desktop/EMAN2_test/

(base) TUAP227:EMAN2_test jl2235$ tiffinfo stack.tif

TIFF Directory at offset 0x8 (8)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x41742 (268098)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x82e7c (536188)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xc45b6 (804278)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x105cf0 (1072368)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x14742a (1340458)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x188b64 (1608548)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x1ca29e (1876638)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x20b9d8 (2144728)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x24d112 (2412818)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x28e84c (2680908)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x2cff86 (2948998)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x3116c0 (3217088)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x352dfa (3485178)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x394534 (3753268)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x3d5c6e (4021358)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x4173a8 (4289448)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x458ae2 (4557538)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x49a21c (4825628)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x4db956 (5093718)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x51d090 (5361808)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x55e7ca (5629898)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x59ff04 (5897988)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x5e163e (6166078)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x622d78 (6434168)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x6644b2 (6702258)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x6a5bec (6970348)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x6e7326 (7238438)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x728a60 (7506528)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x76a19a (7774618)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x7ab8d4 (8042708)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x7ed00e (8310798)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x82e748 (8578888)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x86fe82 (8846978)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x8b15bc (9115068)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x8f2cf6 (9383158)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x934430 (9651248)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x975b6a (9919338)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x9b72a4 (10187428)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x7e4d1f4 (132436468)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x7e8e92e (132704558)

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Bits/Sample: 8

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x7f117a2 (133240738)

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Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x7f52edc (133508828)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x8b15b48 (145840968)

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x8c1b830 (146913328)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x8c5cf6a (147181418)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x98a2a4a (160049738)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0x9966ff8 (160854008)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xb8d98d6 (193829078)

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xb91b010 (194097168)

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xb99de84 (194633348)

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Sample Format: unsigned integer

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xb9df5be (194901438)

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Sample Format: unsigned integer

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbaa3b6c (195705708)

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Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbae52a6 (195973798)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbb6811a (196509978)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbba9854 (196778068)

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Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbbeaf8e (197046158)

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Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbc2c6c8 (197314248)

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbc6de02 (197582338)

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Bits/Sample: 8

Sample Format: unsigned integer

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Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbcaf53c (197850428)

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbcf0c76 (198118518)

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Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

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frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbd323b0 (198386608)

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Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbd73aea (198654698)

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbdb5224 (198922788)

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbdf695e (199190878)

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbe38098 (199458968)

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbe797d2 (199727058)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbebaf0c (199995148)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

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frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbefc646 (200263238)

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbf3dd80 (200531328)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbf7f4ba (200799418)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xbfc0bf4 (201067508)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xc00232e (201335598)

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xc043a68 (201603688)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xc0851a2 (201871778)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

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Color Map: (present)

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xcc89548 (214472008)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xcccac82 (214740098)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xcd8f230 (215544368)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xd9d4d10 (228412688)

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Bits/Sample: 8

Sample Format: unsigned integer

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xda57b84 (228948868)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

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frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xda992be (229216958)

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Bits/Sample: 8

Sample Format: unsigned integer

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Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

channels=1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

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Bits/Sample: 8

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Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe7a334c (242889548)

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Bits/Sample: 8

Sample Format: unsigned integer

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Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

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images=925

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Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe7e4a86 (243157638)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe8261c0 (243425728)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe8678fa (243693818)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe8a9034 (243961908)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe8ea76e (244229998)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe92bea8 (244498088)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe96d5e2 (244766178)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe9aed1c (245034268)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xe9f0456 (245302358)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xea31b90 (245570448)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xea732ca (245838538)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xeab4a04 (246106628)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xeaf613e (246374718)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xeb37878 (246642808)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xeb78fb2 (246910898)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xebba6ec (247178988)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xebfbe26 (247447078)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

TIFF Directory at offset 0xec3d560 (247715168)

Image Width: 512 Image Length: 512

Resolution: 0, 0 pixels/cm

Bits/Sample: 8

Sample Format: unsigned integer

Compression Scheme: None

Photometric Interpretation: palette color (RGB from colormap)

Samples/Pixel: 1

Rows/Strip: 1

Planar Configuration: single image plane

Color Map: (present)

ImageDescription: ImageJ=

hyperstack=true

images=925

channels=1

slices=925

frames=1

Software: OME Bio-Formats 6.6.1

(base) TUAP227:EMAN2_test jl2235$

- Here is the command used for importing this stack

e2import.py --import_tomos --importation=copy --shrink=1

- I am also attaching a screenshot of the browser (e2display.py Browser)
previous to executing the import command in the GUI

-The 16 slices from the imported file are OK, I am just missing the other
910 slices. The imported slices are the first 16 slices

Thanks in advance,

Jaime Llodra

>> <https://groups.google.com/d/msgid/eman2/2edeff1f-6a64-4cd3-8a02-ca3fd772ba8en%40googlegroups.com?utm_medium=email&utm_source=footer>
>> .

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Screen Shot 2021-08-23 at 10.27.13.png

Muyuan Chen

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Aug 23, 2021, 10:24:47 AM8/23/21

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It seems that the problem happens when you it try to convert tiff stack to hdf. I know little about tiff reading in Eman so it is hard to comment much about that. I would try “e2proc2d.py a.tif b.hdf” first and see if it can produce a stack of the right dimension. If not, maybe unstacking the tif, convert to mrc individually then generate hdf stack from that. As long as the input file is correct, I don’t think having a thick volume should trigger any issue in the segmentation.

Muyuan

>>>> Dept. of Biochemistry and Molecular Biology (www.bcm.edu/biochem)
>>>> Academic Director, CryoEM Core (cryoem.bcm.edu)
>>>> Co-Director CIBR Center (www.bcm.edu/research/cibr)
>>>>
>>>>
>>>>
>>>>
>>>>

>>>>> On Aug 19, 2021, at 4:28 AM, Jaime Llodra <jaime...@gmail.com> wrote:
>>>>>
>>>>
>>>>> Dear Group Members,
>>>>>
>>>>> After the tomogram preprocessing the resulting file has only 16 slices. Is this a default setting? Do you know how to get a file with original number of slices?
>>>>>
>>>>> Thanks in advance for your help and support.
>>>>>
>>>>> Jaime Llodra
>>>>>
>>>>
>>>>> --
>>>>> --
>>>>> ----------------------------------------------------------------------------------------------
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>>>>>
>>>>> ---
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>>>>> To view this discussion on the web visit https://groups.google.com/d/msgid/eman2/2edeff1f-6a64-4cd3-8a02-ca3fd772ba8en%40googlegroups.com.

>>>>
>>>
>>>
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>>

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>>
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> <Screen Shot 2021-08-23 at 10.27.13.png>

Jaime Llodra

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Aug 23, 2021, 11:40:36 AM8/23/21

to EMAN2

Hi Muyuan,

I converted the images (they are in DM4 format) using your suggestion. I
got a .hdf stack

I import the stack.hdf file using the Import Tomograms option in the GUI
and I get the following message in the terminal

Mon Aug 23 16:24:05 2021: e2proc3d.py stack.hdf ./tomograms/stack.hdf
--clip 1050,1050,16 --process normalize

the images are 2D - I will now make a 3D image out of the 2D images

Done.

- Is EMAN clipping the stack during the importation process?

- I tried using a multiple of 16 in the number of slices of the stack and
also it did not work

Thanks in advance for any suggestion

Jaime Llodra

> https://groups.google.com/d/msgid/eman2/6e33a854-6f99-443b-a0b4-aca60b30edben%40googlegroups.com
> .

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MuyuanChen

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Aug 23, 2021, 12:02:56 PM8/23/21

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Maybe try doing “e2proc2d.py stack.hdf stack3d.hdf —twod2threed” before importing it as tomogram?

> On Aug 23, 2021, at 10:40 AM, Jaime Llodra <jaime...@gmail.com> wrote:
>
> Hi Muyuan,
>
> I converted the images (they are in DM4 format) using your suggestion. I got a .hdf stack
>
> I import the stack.hdf file using the Import Tomograms option in the GUI and I get the following message in the terminal
>
> Mon Aug 23 16:24:05 2021: e2proc3d.py stack.hdf ./tomograms/stack.hdf --clip 1050,1050,16 --process normalize
>
> the images are 2D - I will now make a 3D image out of the 2D images
>
> Done.
>
>
> - Is EMAN clipping the stack during the importation process?
>
> - I tried using a multiple of 16 in the number of slices of the stack and also it did not work
>
>
>
> Thanks in advance for any suggestion
>
> Jaime Llodra
>
>
> On Monday, August 23, 2021 at 3:24:47 PM UTC+1 g5v...@gmail.com wrote:
> It seems that the problem happens when you it try to convert tiff stack to hdf. I know little about tiff reading in Eman so it is hard to comment much about that. I would try “e2proc2d.py a.tif b.hdf” first and see if it can produce a stack of the right dimension. If not, maybe unstacking the tif, convert to mrc individually then generate hdf stack from that. As long as the input file is correct, I don’t think having a thick volume should trigger any issue in the segmentation.
>
> Muyuan
>

> > On Aug 23, 2021, at 4:33 AM, Jaime Llodra <jaime...@gmail.com <applewebdata://E08C650E-4B59-493F-ABC6-BD8AA097B0BF>> wrote:
> >
> > Dear Members,
> >
> > I am aware that my dataset is not a tomogram. It is a stack of Z slices collected by an SEM. Having said that I have to tell you that years ago I used EMAN2 to process a similar dataset for object classification. This file had a .mrc extension and was processed fine. The current file I am having problems with has a .tif extension and maybe that is problem. I will use the original files which are in the .dm4 format and see how EMAN2 behaves.
> >
> >
> > Here is the output of tiffinfo
> >
> > Last login: Mon Aug 23 09:57:31 on ttys001
> >
> >
> >
> > The default interactive shell is now zsh.
> >
> > To update your account to use zsh, please run `chsh -s /bin/zsh`.
> >

> > For more details, please visit https://support.apple.com/kb/HT208050 <https://support.apple.com/kb/HT208050>.

> >>>> Steven Ludtke, Ph.D. <slu...@bcm.edu <applewebdata://E08C650E-4B59-493F-ABC6-BD8AA097B0BF>> Baylor College of Medicine

> >>>> Charles C. Bell Jr., Professor of Structural Biology

> >>>> Dept. of Biochemistry and Molecular Biology (www.bcm.edu/biochem <http://www.bcm.edu/biochem>)
> >>>> Academic Director, CryoEM Core (cryoem.bcm.edu <http://cryoem.bcm.edu/>)
> >>>> Co-Director CIBR Center (www.bcm.edu/research/cibr <http://www.bcm.edu/research/cibr>)

> >>>>
> >>>>
> >>>>
> >>>>
> >>>>
> >>>>> On Aug 19, 2021, at 4:28 AM, Jaime Llodra <jaime...@gmail.com <applewebdata://E08C650E-4B59-493F-ABC6-BD8AA097B0BF>> wrote:
> >>>>>
> >>>>
> >>>>> Dear Group Members,
> >>>>>
> >>>>> After the tomogram preprocessing the resulting file has only 16 slices. Is this a default setting? Do you know how to get a file with original number of slices?
> >>>>>
> >>>>> Thanks in advance for your help and support.
> >>>>>
> >>>>> Jaime Llodra
> >>>>>
> >>>>
> >>>>> --
> >>>>> --
> >>>>> ----------------------------------------------------------------------------------------------
> >>>>> You received this message because you are subscribed to the Google
> >>>>> Groups "EMAN2" group.

> >>>>> To post to this group, send email to em...@googlegroups.com <applewebdata://E08C650E-4B59-493F-ABC6-BD8AA097B0BF>
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> >>>>> For more options, visit this group at

> >>>>> http://groups.google.com/group/eman2 <http://groups.google.com/group/eman2>

> >>>>>
> >>>>> ---
> >>>>> You received this message because you are subscribed to the Google Groups "EMAN2" group.

> >>>>> To unsubscribe from this group and stop receiving emails from it, send an email to eman2+un...@googlegroups.com <applewebdata://E08C650E-4B59-493F-ABC6-BD8AA097B0BF>.
> >>>>> To view this discussion on the web visit https://groups.google.com/d/msgid/eman2/2edeff1f-6a64-4cd3-8a02-ca3fd772ba8en%40googlegroups.com <https://groups.google.com/d/msgid/eman2/2edeff1f-6a64-4cd3-8a02-ca3fd772ba8en%40googlegroups.com>.

> >>>>
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> > <Screen Shot 2021-08-23 at 10.27.13.png>
>

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Jaime Llodra

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Aug 24, 2021, 10:40:01 AM8/24/21

to EMAN2

Hi Muyuan.

I tried your suggestion and it worked.

I have some additional questions about the file generated after
preprocessing

- The file has 960 slices instead of 921, these are just empty slices it
seems. I do not think it will matter for the subsequent processing.

- There is a problem with the voxel size. The original voxel size is 30nm X
30nm X 90nm and the preprocessed file seems compressed in the Z direction.
I am attaching a screenshot of the main window (e2spt_boxer.py). You can
see how the images are compressed in the Z direction which can be seen on
both the viewer for the XZ and YZ planes. The image of the XY plane
displays the right proportions.
Any idea how to solve this?

Here is the output of the command (--twod2threed) and the output of the
import and preprocessing options in the GUI

(base) TUAP227:EMAN2_test jl2235$ e2proc2d.py stack.hdf stack3d.hdf
--twod2threed

912 images, processing 0-911 stepping by 1

1 images

(base) TUAP227:EMAN2_test jl2235$ ls

DM4_files stack.hdf stack3d.hdf

info stack.tif tomograms

(base) TUAP227:EMAN2_test jl2235$ e2projectmanager.py

NOT Writing notes, ppid=-2

Tue Aug 24 12:14:43 2021: e2proc3d.py stack3d.hdf ./tomograms/stack3d.hdf
--clip 1050,1050,960 --process normalize

Done.

NOT Writing notes, ppid=-2

1/1NOT Writing notes, ppid=-2

Thanks in advance for your help and support

Screen Shot 2021-08-24 at 13.11.12.png

Ludtke, Steven J.

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Aug 24, 2021, 10:56:43 AM8/24/21

to em...@googlegroups.com

There is no mechanism in CryoEM which would produce files with anisotropic sampling, so this isn't something we really deal with on a regular basis. Having said that, the deep learning segmentation tool works on X-Y slices, so it doesn't really matter that the Z sampling is different. Certainly it is possible to rescale the volume so the Z sampling is the same as X and Y. The question then is whether you want to reduce the X-Y resolution to 90, or increase the Z sampling to 30 (which would make a very large file!)?

--------------------------------------------------------------------------------------
Steven Ludtke, Ph.D. <slu...@bcm.edu<mailto:slu...@bcm.edu>> Baylor College of Medicine

Charles C. Bell Jr., Professor of Structural Biology
Dept. of Biochemistry and Molecular Biology (www.bcm.edu/biochem<http://www.bcm.edu/biochem>)

Academic Director, CryoEM Core (cryoem.bcm.edu<http://cryoem.bcm.edu>)

Co-Director CIBR Center (www.bcm.edu/research/cibr<http://www.bcm.edu/research/cibr>)

On Aug 24, 2021, at 9:40 AM, Jaime Llodra <jaime...@gmail.com<mailto:jaime...@gmail.com>> wrote:

Hi Muyuan.

I tried your suggestion and it worked.

I have some additional questions about the file generated after preprocessing

- The file has 960 slices instead of 921, these are just empty slices it seems. I do not think it will matter for the subsequent processing.

- There is a problem with the voxel size. The original voxel size is 30nm X 30nm X 90nm and the preprocessed file seems compressed in the Z direction. I am attaching a screenshot of the main window (e2spt_boxer.py). You can see how the images are compressed in the Z direction which can be seen on both the viewer for the XZ and YZ planes. The image of the XY plane displays the right proportions.
Any idea how to solve this?

Here is the output of the command (--twod2threed) and the output of the import and preprocessing options in the GUI

(base) TUAP227:EMAN2_test jl2235$ e2proc2d.py stack.hdf stack3d.hdf --twod2threed

912 images, processing 0-911 stepping by 1

1 images

(base) TUAP227:EMAN2_test jl2235$ ls

DM4_files stack.hdf stack3d.hdf

info stack.tif tomograms

(base) TUAP227:EMAN2_test jl2235$ e2projectmanager.py

NOT Writing notes, ppid=-2

Tue Aug 24 12:14:43 2021: e2proc3d.py stack3d.hdf ./tomograms/stack3d.hdf --clip 1050,1050,960 --process normalize

Done.

NOT Writing notes, ppid=-2

1/1NOT Writing notes, ppid=-2

Thanks in advance for your help and support

> >>>> Dept. of Biochemistry and Molecular Biology (www.bcm.edu/biochem<http://www.bcm.edu/biochem> <http://www.bcm.edu/biochem>)
> >>>> Academic Director, CryoEM Core (cryoem.bcm.edu<http://cryoem.bcm.edu/> <http://cryoem.bcm.edu/>)
> >>>> Co-Director CIBR Center (www.bcm.edu/research/cibr<http://www.bcm.edu/research/cibr> <http://www.bcm.edu/research/cibr>)

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<Screen Shot 2021-08-24 at 13.11.12.png>

Jaime Llodra

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Aug 24, 2021, 11:40:02 AM8/24/21

to EMAN2

I think you are right, EMAN2 works on XY slices. I'll leave it as it is and
continue my work.

Thanks Steve and Muyuan, you have been very helpful

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