Hi Javad,
Using a B-spline interpolator can be problematic when resampling binary masks after registration because B-spline interpolation is a continuous function and may generate values between 0 and 1 for pixels that should be strictly binary (i.e., either 0 or 1). This can lead to the introduction of intermediate values between 0 and 1, which can cause errors when the binary mask is used for subsequent analyses or processing steps.
For example, if a binary mask is being used to delineate a region of interest (ROI), intermediate values introduced by the B-spline interpolation can cause the ROI to be inaccurately defined or even split into multiple regions. Additionally, if the binary mask is being used for segmentation or thresholding, the introduction of intermediate values can cause errors or inaccuracies in the resulting segmentation or thresholding.
To avoid these issues, other interpolation methods, such as nearest-neighbor interpolation should be used when resampling binary masks. These methods ensure that the resampled values are either 0 or 1, which preserves the binary nature of the mask and reduces the likelihood of errors in subsequent processing steps.
To use this with your current schema, set (FinalBSplineInterpolationOrder 3) --> (FinalBSplineInterpolationOrder 0)
Best of luck,
Heath