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CV
Jan 31, 2016, 3:59:26 PM1/31/16
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Hi,
I've been using Deconseq to see how many reads of an Illumina HiSeq run (100bp, so not that long ...) could be attributed to a parasite living withing my organism.
I've got pretty different results from Deconseq run compared to just mapping the reads to the parasite reference genome using 'bwa mem' or 'bwaSW' (almost 10 fold higher in Deconseq).
Are the Deconseq results trustworthy or not? I was a bit surprised to see such huge differences ...
Best
CV
I've been using Deconseq to see how many reads of an Illumina HiSeq run (100bp, so not that long ...) could be attributed to a parasite living withing my organism.
I've got pretty different results from Deconseq run compared to just mapping the reads to the parasite reference genome using 'bwa mem' or 'bwaSW' (almost 10 fold higher in Deconseq).
Are the Deconseq results trustworthy or not? I was a bit surprised to see such huge differences ...
Best
CV
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