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Letícia Cavalcante
May 17, 2018, 4:09:49 PM5/17/18
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Hello, everyone,
We’re using PRINSEQ to make the quality control in some publicly available metagenomic sequences but we are facing some issues to clean paired-end samples from SRA. After running Prinseq using the following line:
perl ./prinseq-lite.pl -verbose -fastq /home/lcavalcante/corais/SRR5215455_pass_1.fastq -fastq2 /home/DIR/SRR5215455_pass_2.fastq -min_len 100 -ns_max_p 1 -out_format 1 -out_good /home/DIR/SRR5215455_good
The result was:
Estimate size of input data for status report (this might take a while for large files)
done
Parse and process input data
status: 99 %
ERROR: The number of bases and quality scores are not the same for sequence "SRR5215455.64248690_right".
Is it possible that problem is in our command line?
We’re using PRINSEQ to make the quality control in some publicly available metagenomic sequences but we are facing some issues to clean paired-end samples from SRA. After running Prinseq using the following line:
perl ./prinseq-lite.pl -verbose -fastq /home/lcavalcante/corais/SRR5215455_pass_1.fastq -fastq2 /home/DIR/SRR5215455_pass_2.fastq -min_len 100 -ns_max_p 1 -out_format 1 -out_good /home/DIR/SRR5215455_good
The result was:
Estimate size of input data for status report (this might take a while for large files)
done
Parse and process input data
status: 99 %
ERROR: The number of bases and quality scores are not the same for sequence "SRR5215455.64248690_right".
Is it possible that problem is in our command line?
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