I wanted to filter my paired end fastq files according to their quality score. Can anyone provide me some example of the command lines about this topic.
E.G. to remove 'N' from the sequence I am using this line:
perl /Users/ZILLURRAHMAN/Desktop/PhD/Filter/prinseq-lite-0.20.4/prinseq-lite.pl -verbose -fastq SRR901607_1.fastq -fastq2 SRR901607_2.fastq -ns_max_n 0 -out_good strain_gi_no_ns -out_bad strain_gi_with_ns
Now if I want to remove sequence with low quality score what would be my possible commands. Is there any example?