number of joined reads

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Erin

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Jan 9, 2015, 12:05:14 PM1/9/15
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Hello,

I used fastq to join forward and reverse reads from Illumina, below: 

# Join forward and reverse reads

$ ./fastq-join Undetermined_S0_L001_R1_001.fastq Undetermined_S0_L001_R2_001.fastq -o joined.fastq

## FASTQ JOIN STATS
## Total reads: 16009546
## Total joined: 10296305
## Average join len: 49.64
## Stdev join len: 5.43

Normally I would expect to have a total number of joined reads that is half (or less) of the original read pool.
However, I got ~60% of the total reads, joined (~10 million out of ~16 million).
Does this reflect the potentially poor quality of the reverse reads?
Or is there another explanation?

Thanks for your help!
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