Hello,
I used fastq to join forward and reverse reads from Illumina, below:
# Join forward and reverse reads
$ ./fastq-join Undetermined_S0_L001_R1_001.fastq Undetermined_S0_L001_R2_001.fastq -o joined.fastq
## FASTQ JOIN STATS
## Total reads: 16009546
## Total joined: 10296305
## Average join len: 49.64
## Stdev join len: 5.43
Normally I would expect to have a total number of joined reads that is half (or less) of the original read pool.
However, I got ~60% of the total reads, joined (~10 million out of ~16 million).
Does this reflect the potentially poor quality of the reverse reads?
Or is there another explanation?
Thanks for your help!