Dear Erik,
Thank you so much for writing fastq-multx as it is fantastic and quick for de-multiplexing my RNAseq data. My question is about whether or not I can get it to recognise barcodes that are not at the start of the read. For example, my *fastq.gz file consisted of 9 samples with barcodes listed below. 7/9 samples decoded perfectly and I have the associated files. However for iCLIP3A and iCLIP3B, I've noticed that the barcode is not at the 5' end of the read so it is something like NNNGGCA.... and NNNTTAA.......
I've tried giving it a barcode file that has the index as "NNNGGCA" and "NNNTTAA" but this doesn't seem to work. I know you have a "mismatch" option but I'm assuming this means mismatch WITHIN the barcode not leading up to the barcode.
IgG1 GAT
IgG2 GGC
iCLIP1A AAC
iCLIP2A AGT
iCLIP3A GGCA
IgG3 TTC
iCLIP1B CCC
iCLIP2B TCT
iCLIP3B TTAA
If you have any suggestions as to how I can deal with this, they will be greatly appreciated.
Cheers,
Manasa